Carlsson Lena, Ronquist Gunnar, Eliasson Rune, Egberg Nils, Larsson Anders
Department of Medical Sciences, University Hospital, Uppsala, Sweden.
Int J Androl. 2006 Apr;29(2):331-8. doi: 10.1111/j.1365-2605.2005.00601.x.
Summary Prostasomes are prostate-derived organelles in seminal plasma exhibiting pluripotent properties to facilitate the fertilization process. Seminal prostasome concentration, size distribution and expression of the prostasomal surface antigens CD10, CD13, CD26 and CD59 were examined by flow cytometry. The study group consisted of 79 men with involuntary infertility. Very strong correlations existed between the prostasome expressions of the different CD markers. Significant correlations between prostasome concentration and CD molecules were weak or lacking. Further, no or weak relationships were observed between the prostasomal CD markers and sperm morphology, seminal fructose, neutral alpha-glucosidase activity, zinc and tumour necrosis factor alpha concentrations. Flow cytometry is a practical way to study prostasomes in seminal fluid without prior separation. This is a new technique for evaluation of the role of prostasomes and their functions in male reproductive physiology.
摘要 前列腺小体是精液中源自前列腺的细胞器，具有多能特性以促进受精过程。通过流式细胞术检测了精液中前列腺小体的浓度、大小分布以及前列腺小体表面抗原CD10、CD13、CD26和CD59的表达。研究组由79名非自愿性不育男性组成。不同CD标志物的前列腺小体表达之间存在非常强的相关性。前列腺小体浓度与CD分子之间的显著相关性较弱或不存在。此外，未观察到前列腺小体CD标志物与精子形态、精液果糖、中性α-葡萄糖苷酶活性、锌和肿瘤坏死因子α浓度之间存在关系或仅有微弱关系。流式细胞术是一种无需事先分离即可研究精液中前列腺小体的实用方法。这是一种评估前列腺小体及其在男性生殖生理学中的功能作用的新技术。
