Carlsson Lena, Nilsson Ove, Larsson Anders, Stridsberg Mats, Sahlén Göran, Ronquist Gunnar
Department of Medical Sciences, Clinical Chemistry Unit, University Hospital, Uppsala, Sweden.
Prostate. 2003 Mar 1;54(4):322-30. doi: 10.1002/pros.10189.
Prostasomes are organelles secreted by human prostate epithelial cells and appear freely in semen. Most prostasomal functional and biochemical characteristics have been settled on basis of seminal prostasomes (SP).
Three types of prostasomes, viz., from seminal plasma, prostate gland (native), and prostate cancer bone metastasis, were isolated and compared for their biochemical and functional characteristics.
Flow cytometry showed that all three types of prostasomes had a similar size distribution and electrophoresis revealed close similarities in banding pattern. Also, a high cholesterol/phospholipid ratio and some marker enzymes were distinctive common features. The unusual chromogranin B (CgB)/chromogranin A (CgA) ratio with CgB in abundance over CgA, previously found only in SP, was also valid for the native (NP) and metastasis-derived prostasomes (MP). The latter prostasome type contained a 10-fold higher amount of CgA compared to the others. SP and MP showed the same promotive effect on sperm forward motility, whereas NP did not. All three prostasome types exhibited an antibacterial activity against Bacillus megaterium.
Since the three prostasome types were isolated from different environments, our data support the view that the common characteristics were prostasome-genuine.
前列腺小体是人类前列腺上皮细胞分泌的细胞器，可自由存在于精液中。大多数前列腺小体的功能和生化特性是基于精浆前列腺小体（SP）确定的。
分离出三种类型的前列腺小体，即来自精浆、前列腺（天然）和前列腺癌骨转移灶的前列腺小体，并比较它们的生化和功能特性。
流式细胞术显示，所有三种类型的前列腺小体具有相似的大小分布，电泳显示条带模式非常相似。此外，高胆固醇/磷脂比率和一些标记酶是明显的共同特征。以前仅在SP中发现的异常嗜铬粒蛋白B（CgB）/嗜铬粒蛋白A（CgA）比率，即CgB含量高于CgA，对于天然前列腺小体（NP）和转移来源的前列腺小体（MP）也同样适用。后一种类型的前列腺小体所含CgA的量比其他类型高10倍。SP和MP对精子向前运动具有相同的促进作用，而NP则没有。所有三种类型的前列腺小体均对巨大芽孢杆菌表现出抗菌活性。
由于这三种类型的前列腺小体是从不同环境中分离出来的，我们的数据支持这样一种观点，即这些共同特征是前列腺小体所固有的。
