School of Bioscience and Veterinary Medicine, Università Di Camerino, Via Circonvallazione 93/95, 62024, Matelica (Macerata), Italy.
Department of Animal Medicine, Production and Health Università Degli Studi Di Padova, Agripolis Viale Dell'Università - 35020 Legnaro, Padua, Italy.
Vet Res Commun. 2024 Feb;48(1):357-366. doi: 10.1007/s11259-023-10202-1. Epub 2023 Sep 14.
Canine seminal plasma is a complex fluid containing proteins, peptides, enzymes, hormones as well as extracellular vesicles that are involved in many physiological and pathological processes including reproduction. We examined the expression of the extracellular vesicles surface antigens Aminopeptidase-N (CD13) and Dipeptidyl peptidase IV (CD26) by flow cytometry. For this study, third fraction of the ejaculate, from fertile adult male German Shepherd dogs, was manually collected twice, two days apart. FACS analyses revealed that CD13 and CD26 are co-expressed on the 69.3 ± 3.7% of extracellular vesicles and only a 2.0 ± 0.5% of extracellular vesicles express CD26 alone. On the other hand, 28.6 ± 3.6% of seminal EVs express CD13 alone. Our results agree with the hypothesis that CD26 needs to be co-expressed with other signal-transducing molecules, while CD13, can perform functions independently of the presence or co-expression of CD26. The results obtained in normal fertile dogs could represent physiological expression of these enzymes. Therefore, it would be interesting to carry out further studies to evaluate the expression of CD13 and CD26 on extracellular vesicles as biomarker for prostate pathological condition in dogs.
犬精液是一种含有蛋白质、肽、酶、激素以及参与许多生理和病理过程（包括生殖）的细胞外囊泡的复杂液体。我们通过流式细胞术检查了细胞外囊泡表面抗原氨肽酶-N（CD13）和二肽基肽酶 IV（CD26）的表达。在这项研究中，从成年德国牧羊犬的第三次射精部分手动采集两次，间隔两天。FACS 分析显示，CD13 和 CD26 在 69.3 ± 3.7%的细胞外囊泡上共同表达，只有 2.0 ± 0.5%的细胞外囊泡单独表达 CD26。另一方面，28.6 ± 3.6%的精子 EVs 单独表达 CD13。我们的结果与 CD26 需要与其他信号转导分子共同表达的假设一致，而 CD13 可以在不依赖 CD26 存在或共同表达的情况下发挥功能。在正常生育犬中获得的结果可能代表这些酶的生理表达。因此，进一步研究评估 CD13 和 CD26 在犬前列腺病理条件下作为细胞外囊泡生物标志物的表达将是有趣的。
