Hoebler C, Gaudier E, De Coppet P, Rival M, Cherbut C
Unité des Fonctions Digestives et de Nutrition Humaine, BP 71627, 44316, Nantes Cedex 3, France.
Dig Dis Sci. 2006 Feb;51(2):381-9. doi: 10.1007/s10620-006-3142-y.
Colonic mucosal protection is provided by mucous gel, mainly composed of secreted (Muc2) and membrane-bound (Muc1, Muc3, Muc4) mucins. Our aim was to determine the expression profile of secreted and membrane-bound mucins in experimental dextran sulfate sodium (DSS)-induced colitis. Acute colitis was induced in Balb/C mice by oral administration of 1.0% DSS (5 days) and chronic colitis was maintained by subsequent 0.15% DSS treatment (28 days). Clinical symptoms (mortality, weight gain), stool scores, and MPO activity confirmed the inflammatory state in the two phases of colitis. Muc2 gene expression was not modified by colitis, whereas Muc3 gene expression was increased (x2) only in the cecum and the distal colon of mice after acute colitis. Muc1 and Muc4 mRNA levels were more significantly increased in the cecum (x8-10) than in colonic segments (x4) after acute colitis. TFF3 involved in mucosal repair was up-regulated during colitis induction. These results indicate that Muc and TFF3 genes are regulated early in inflammation and suggest that their mRNA levels could be used as early markers of inflammation.
结肠黏膜保护由黏液凝胶提供，黏液凝胶主要由分泌型（Muc2）和膜结合型（Muc1、Muc3、Muc4）黏蛋白组成。我们的目的是确定在实验性葡聚糖硫酸钠（DSS）诱导的结肠炎中分泌型和膜结合型黏蛋白的表达谱。通过口服1.0% DSS（5天）诱导Balb/C小鼠发生急性结肠炎，并通过随后0.15% DSS治疗（28天）维持慢性结肠炎。临床症状（死亡率、体重增加）、粪便评分和MPO活性证实了结肠炎两个阶段的炎症状态。Muc2基因表达未因结肠炎而改变，而Muc3基因表达仅在急性结肠炎后小鼠的盲肠和远端结肠中增加（x2）。急性结肠炎后，盲肠中Muc1和Muc4 mRNA水平的增加（x8 - 10）比结肠段（x4）更显著。参与黏膜修复的TFF3在结肠炎诱导期间上调。这些结果表明，Muc和TFF3基因在炎症早期受到调控，并表明它们的mRNA水平可作为炎症的早期标志物。
