Martins Luisa Caricio, de Oliveira Corvelo Tereza Cristina, Oti Henrique Takeshi, do Socorro Pompeu Loiola Rosane, Aguiar Delia Cristina Figueira, dos Santos Barile Katarine Antonia, do Amaral Renata Kelly Costa, Barbosa Hivana Patricia Melo, Fecury Amanda Alves, de Souza Juciclayton Tavares
Immunogenetics Laboratory , Department of Genetics, Federal University of Pará, Brazil.
World J Gastroenterol. 2006 Feb 21;12(7):1120-4. doi: 10.3748/wjg.v12.i7.1120.
To investigate the ABH and Lewis antigen expression in erythrocytes, saliva and gastric epithelium, as well as the association between H pylori and the presence of gastric epithelial lesions.
The distribution of ABH and Lewis blood group antigens in erythrocytes, saliva and gastric mucosa of H pylori-infected gastric ulcer patients was analyzed. Forty-two patients with gastric ulcer were studied, and fifty healthy individuals were used as control group. The blood group antigens were determined by direct hemagglutination, dot-ELISA and immunohistochemical methods in erythrocytes, saliva and gastric mucosa specimens, respectively. Diagnosis for H pylori infection was performed by conventional optical microscopy and ELISA.
A higher seroprevalence of IgG H pylori specific antibodies was observed in gastric ulcer patients (90%) compared to the control group (60%). We observed a significant increase of phenotypes O, A2 and Lewis b in H pylori-infected patients. The expression of these antigens had progressive alterations in areas of ulcerous lesions and intestinal metaplasia.
ABH and Lewis blood group antigens are a good indicator for cellular alterations in the gastric epithelium.
研究红细胞、唾液及胃上皮中ABH和Lewis抗原的表达情况，以及幽门螺杆菌与胃上皮病变存在之间的关联。
分析幽门螺杆菌感染的胃溃疡患者红细胞、唾液及胃黏膜中ABH和Lewis血型抗原的分布。研究了42例胃溃疡患者，并以50名健康个体作为对照组。分别采用直接血凝法、斑点酶联免疫吸附测定法和免疫组织化学方法检测红细胞、唾液及胃黏膜标本中的血型抗原。通过传统光学显微镜和酶联免疫吸附测定法进行幽门螺杆菌感染诊断。
与对照组（60%）相比，胃溃疡患者中幽门螺杆菌特异性IgG抗体的血清阳性率更高（9例）。我们观察到幽门螺杆菌感染患者中O型、A2型和Lewis b型的表型显著增加。这些抗原的表达在溃疡病变和肠化生区域有渐进性改变。
ABH和Lewis血型抗原是胃上皮细胞改变的良好指标。
