Ansari Shazia Akbar, Khan Adnan, Khan Taseer Ahmed, Raza Yasir, Syed Sofia Ali, Akhtar Syed Shakeel, Kazmi Shahana Urooj
Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, Karachi, Pakistan.
Trop Med Int Health. 2015 Jan;20(1):115-9. doi: 10.1111/tmi.12401. Epub 2014 Oct 16.
A and B blood group antigens are fucosylated carbohydrate present on human erythrocytes and body secretions. Their presence in body secretions depends on the expression of a dominant allele of secretor gene FUT2 and is correlated with susceptibility to various infectious and non-infectious diseases. We investigated the correlation of blood group and ABH antigen secretion with Helicobacter pylori infection and gastroduodenal symptoms and analysed the distribution of babA gene among ABH secretors and non-secretors.
Two hundred and ninety patients who underwent gastroduodenal endoscopy during 2011 to 2012 participated. Gastric biopsy, saliva and blood samples were obtained from every patient. Gastric biopsies were subjected to rapid urease test and PCR for the detection of H. pylori and babA gene. Blood grouping and ABH antigens secretions were determined by Lewis blood group phenotyping and haemagglutination inhibition test.
50.34% of patients were ABH antigen secretors and 45.51% non-secretors. Distribution analysis of blood group revealed that 40 blood group B, 67 blood group A 20 blood group O and 19 blood group AB patients secreted ABH antigens in saliva. Fifty-six blood group O, 19 blood group B, 32 blood group A and 17 blood group AB patients were non-secretors. Gastroduodenal complaints were common among non-secretors. Sixty-two percent of patients with a combination of duodenal ulcer and gastro-oesophageal reflux and 54% of patients with gastritis were non-secretors. Of 290 samples, 31.02% were positive for H. pylori. Thirty percent of these tested positive for babA gene; the majority belonged to non-secretor blood group O.
Our results suggest that the infection of H. pylori is correlated with ABO blood groups and blood group antigens secretion in body fluids.
A和B血型抗原是存在于人类红细胞和身体分泌物上的岩藻糖基化碳水化合物。它们在身体分泌物中的存在取决于分泌基因FUT2显性等位基因的表达，并与对各种感染性和非感染性疾病的易感性相关。我们研究了血型和ABH抗原分泌与幽门螺杆菌感染及胃十二指肠症状的相关性，并分析了ABH分泌者和非分泌者中babA基因的分布情况。
2011年至2012年期间接受胃十二指肠内镜检查的290例患者参与了研究。从每位患者身上采集胃活检组织、唾液和血液样本。胃活检组织进行快速尿素酶试验和PCR检测幽门螺杆菌及babA基因。通过Lewis血型分型和血凝抑制试验确定血型和ABH抗原分泌情况。
50.34%的患者为ABH抗原分泌者，45.51%为非分泌者。血型分布分析显示，40例B型血、67例A型血、20例O型血和19例AB型血患者唾液中分泌ABH抗原。56例O型血、19例B型血、32例A型血和17例AB型血患者为非分泌者。胃十二指肠症状在非分泌者中较为常见。十二指肠溃疡合并胃食管反流患者中有62%、胃炎患者中有54%为非分泌者。在290份样本中，31.02%幽门螺杆菌检测呈阳性。其中30%检测出babA基因呈阳性；大多数属于非分泌者O型血。
我们的结果表明，幽门螺杆菌感染与ABO血型及体液中血型抗原分泌相关。
