Ikehara Y, Nishihara S, Kudo T, Hiraga T, Morozumi K, Hattori T, Narimatsu H
Division of Cell Biology, Institute of Life Science, Soka University, Hachioji, Tokyo, Japan.
Glycoconj J. 1998 Aug;15(8):799-807. doi: 10.1023/a:1006964016344.
Immunohistochemical staining showed an aberrant expression of Le(a) antigen in the intestinal metaplastic glands of the gastric mucosa of secretors, as reported by others. In this study, we have demonstrated for the first time that the Lewis enzyme is well colocalized with Le(a) antigen, indicating that the Lewis enzyme is responsible for Le(a) antigen synthesis in the gastric mucosa. The staining intensity of the Lewis enzyme was much stronger in the cells with intestinal metaplasia than the cells without metaplasia, regardless of the secretor status. The amount of transcript of the Lewis gene was related to the degree of metaplasia; i.e., the more severe the metaplastic change was, the more abundantly the transcripts of the Lewis gene were expressed. This augmentation of the Lewis enzyme in metaplastic tissues was also confirmed by Western blotting analysis using a specific antibody against the Lewis enzyme. We conclude that intestinal metaplastic change of gastric mucosa is usually accompanied by a marked augmentation of the Lewis enzyme expression, which results in the enhanced expression of Le(a) antigens, particularly in secretors.
免疫组织化学染色显示，如其他人所报道的，分泌型个体胃黏膜肠化生腺体中Le(a)抗原表达异常。在本研究中，我们首次证明Lewis酶与Le(a)抗原共定位良好，表明Lewis酶负责胃黏膜中Le(a)抗原的合成。无论分泌型状态如何，肠化生细胞中Lewis酶的染色强度均明显强于未化生细胞。Lewis基因的转录本数量与化生程度相关，即化生改变越严重，Lewis基因转录本表达越丰富。使用针对Lewis酶的特异性抗体进行的蛋白质印迹分析也证实了化生组织中Lewis酶的增加。我们得出结论，胃黏膜的肠化生改变通常伴随着Lewis酶表达的显著增加，这导致Le(a)抗原表达增强，尤其是在分泌型个体中。
