Appl Environ Microbiol. 1996 Mar;62(3):809-14. doi: 10.1128/aem.62.3.809-814.1996.
The conversion by a methanogenic consortium of phenol into phenylalanine, with benzoic and phenylpropionic acid as intermediates, was investigated. When (sup14)C-labelled phenol was fed to the consortium, the radioactivity was mostly transferred into methane and CO(inf2), but 4% of the radioactivity was found in the water fraction after extraction of the culture medium with an organic solvent. Utilization of labelled compounds and analysis by gas chromatography coupled with mass spectrometry revealed that a fraction of the benzoic acid produced was transformed into 3-phenylpropionic acid. When fully (sup13)C-labelled acetic acid was fed to the consortium, the labels were incorporated at the 1 and 2 positions of 3-phenylpropionic acid. When deuterium-labelled 3-phenylpropionic acid was fed to the consortium, part of the phenylalanine of the biomass was labelled. These metabolic transformations are reversible, since deuterium-labelled phenylalanine generated labelled 3-phenylpropionic acid. Cinnamic acid was also transformed into 3-phenylpropionic acid.
用产甲烷菌混合物将苯酚转化为苯丙氨酸，其中苯甲酸和苯丙酸为中间产物，对此进行了研究。当将 (sup14)C 标记的苯酚喂给混合物时，放射性主要转移到甲烷和 CO(inf2)中，但在用有机溶剂提取培养基后，在水相中发现 4%的放射性。利用标记化合物并通过气相色谱-质谱联用进行分析表明，产生的苯甲酸的一部分转化为 3-苯基丙酸。当完全 (sup13)C 标记的乙酸喂给混合物时，标记被掺入 3-苯基丙酸的 1 和 2 位。当将氘标记的 3-苯基丙酸喂给混合物时，生物量中的一部分苯丙氨酸被标记。这些代谢转化是可逆的，因为氘标记的苯丙氨酸生成了标记的 3-苯基丙酸。肉桂酸也转化为 3-苯基丙酸。
