Technical Resources, Inc., and Microbial Ecology and Biotechnology Branch, Environmental Research Laboratory, Sabine Island, U.S. Environmental Protection Agency, Gulf Breeze, Florida 32561.
Appl Environ Microbiol. 1989 Jun;55(6):1472-6. doi: 10.1128/aem.55.6.1472-1476.1989.
Four methanogenic consortia which degraded 2-chlorophenol, 3-chlorophenol, 2-chlorobenzoate, and 3-chlorobenzoate, respectively, and one nitrate-reducing consortium which degraded 3-chlorobenzoate were characterized. Degradative activity in these consortia was maintained by laboratory transfer for over 2 years. In the methanogenic consortia, the aromatic ring was dechlorinated before mineralization to methane and carbon dioxide. After dechlorination, the chlorophenol consortia converted phenol to benzoate before mineralization. All methanogenic consortia degraded both phenol and benzoate. The 3-chlorophenol and 3-chlorobenzoate consortia also degraded 2-chlorophenol. No other cross-acclimation to monochlorophenols or monochlorobenzoates was detected in the methanogenic consortia. The consortium which required nitrate for the degradation of 3-chlorobenzoate degraded benzoate and 4-chlorobenzoate anaerobically in the presence of KNO(3), but not in its absence. This consortium also degraded benzoate, but not 3-chlorobenzoate, aerobically.
分别降解 2-氯苯酚、3-氯苯酚、2-氯苯甲酸和 3-氯苯甲酸的 4 个产甲烷菌群和一个还原硝酸盐的菌群被鉴定。这些菌群中的降解活性通过实验室传代培养维持了 2 年以上。在产甲烷菌群中，芳香环在矿化为甲烷和二氧化碳之前被脱氯。脱氯后，氯酚菌群在矿化前将苯酚转化为苯甲酸。所有产甲烷菌群都能降解苯酚和苯甲酸。3-氯苯酚和 3-氯苯甲酸菌群也能降解 2-氯苯酚。在产甲烷菌群中未检测到对单氯苯酚或单氯苯甲酸的其他交叉驯化。需要硝酸盐才能降解 3-氯苯甲酸的菌群在有 KNO3存在的情况下能够厌氧降解苯甲酸和 4-氯苯甲酸，但在没有 KNO3的情况下则不能。该菌群还能在有氧条件下降解苯甲酸，但不能降解 3-氯苯甲酸。
