基于光纤的色素单细胞原位光谱学。
Optical fiber-based in situ spectroscopy of pigmented single colonies.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3339-43. doi: 10.1128/aem.62.9.3339-3343.1996.
Abstract
We have adapted a commercially available fiber-optic spectroradiometer with diode array detection to record reflection and absorption spectra from single, 1-mm-diameter bacterial colonies. A careful assessment of the performance of the spectroradiometer for this application is reported. In a model study employing colonies from various phototrophic bacteria, we show that the reflectance spectra are reliable within the range of 450 to 820 nm, whereas the transmission spectra yield accurate peak intensities and absorption maxima from 400 to 900 nm. For screening of populations of about 10(sup4) colonies, fiber-optic transmission spectroscopy provides an attractive and inexpensive alternative to present techniques based on charge-coupled device imaging technology.
摘要
我们对商业上可用的光纤光谱仪与二极管阵列探测器进行了改造，以记录单 1 毫米直径细菌菌落的反射和吸收光谱。报告了对该应用光谱仪性能的仔细评估。在使用来自各种光养细菌的菌落的模型研究中，我们表明在 450 到 820nm 的范围内，反射率光谱是可靠的，而透射光谱则能从 400 到 900nm 精确地获得峰强度和吸收最大值。对于约 10(sup4)个菌落的群体筛选，光纤传输光谱学为基于电荷耦合器件成像技术的现有技术提供了一种有吸引力和经济实惠的替代方案。
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本文引用的文献
1
Optimization of the Sistrom Culture Medium for Large-Scale Batch Cultivation of Rhodospirillum rubrum under Semiaerobic Conditions with Maximal Yield of Photosynthetic Membranes.优化 Sistrom 培养基，在半需氧条件下进行大规模批量培养红假单胞菌，以获得最大量的光合膜。
Appl Environ Microbiol. 1994 May;60(5):1698-700. doi: 10.1128/aem.60.5.1698-1700.1994.
2
Analysis of Fungal Pellets by UV-Visible Spectrum Diffuse Reflectance Spectroscopy.运用紫外-可见漫反射光谱分析真菌颗粒。
Appl Environ Microbiol. 1993 Dec;59(12):4253-60. doi: 10.1128/aem.59.12.4253-4260.1993.
3
Absorption spectrophotometry of turbid suspensions: a method of correcting for large systematic distortions.浑浊悬浮液的吸收分光光度法：一种校正大的系统偏差的方法。
Arch Biochem Biophys. 1962 Aug;98:274-85. doi: 10.1016/0003-9861(62)90184-4.
4
A requirement for sodium in the growth of Rhodopseudomonas spheroides.球形红假单胞菌生长对钠的需求。
J Gen Microbiol. 1960 Jun;22:778-85. doi: 10.1099/00221287-22-3-778.
5
Selective scattering of light by pigments in vivo.体内色素对光的选择性散射。
Arch Biochem Biophys. 1959 Oct;84:428-41. doi: 10.1016/0003-9861(59)90605-8.
6
Green fluorescent protein as a marker for gene expression.绿色荧光蛋白作为基因表达的标志物。
Science. 1994 Feb 11;263(5148):802-5. doi: 10.1126/science.8303295.
7
In situ determination of the reduction levels of cytochromes b and c in growing bacteria: a case study with N2-fixing Azorhizobium caulinodans.
Anal Biochem. 1993 Oct;214(1):149-55. doi: 10.1006/abio.1993.1470.
8
Imaging sequence space.
Nature. 1994 May 5;369(6475):79-80. doi: 10.1038/369079a0.
9
Resonance light scattering: a new technique for studying chromophore aggregation.
Science. 1995 Aug 18;269(5226):935-9. doi: 10.1126/science.7638615.
10
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