Sajjaphan Kannika, Shapir Nir, Wackett Lawrence P, Palmer Michael, Blackmon Barbara, Tomkins Jeff, Sadowsky Michael J
Department of Soil, Water, and Climate, Center for Microbial and Plant Genomics, 439 Borlaug Hall, 1991 Upper Buford Cir., University of Minnesota, St. Paul, MN 55108, USA.
Appl Environ Microbiol. 2004 Jul;70(7):4402-7. doi: 10.1128/AEM.70.7.4402-4407.2004.
Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. Hybridization studies localized trzN, atzB, and atzC to a 380-kb plasmid in A. aurescens strain TC1.
金色节杆菌菌株TC1通过TrzN、AtzB和AtzC将阿特拉津代谢为氰尿酸。一个160 kb细菌人工染色体克隆的完整序列表明,trzN、atzB和atzC在金色节杆菌基因组上是连锁的。TrzN、AtzB和AtzC在大肠杆菌中显示出功能。杂交研究将trzN、atzB和atzC定位到金色节杆菌菌株TC1的一个380 kb质粒上。