Yanze-Kontchou C, Gschwind N
Environmental Process Technology, Ciba-Geigy AG, CH-4133 Schweizerhalle, Switzerland.
Appl Environ Microbiol. 1994 Dec;60(12):4297-302. doi: 10.1128/aem.60.12.4297-4302.1994.
Strain YAYA6 was isolated from a mixed microbial community that was growing on atrazine as a sole carbon source and formed quantitative amounts of chloride and nitrate. This strain was identified as a member of the true pseudomonad group (RNA group I) and was given the designation DMS 93-99. The growth yield when atrazine was the sole carbon and nitrogen source was 80 g (dry weight) of cells per mol of atrazine, and the cell doubling time was around 11 h. Approximately 20% of [U-ring 14C]atrazine was mineralized during primary degradation of atrazine. After atrazine disappeared from the culture supernatant, mineralization continued until the level of mineralization was more than 50%. Under different experimental conditions 10% of the atrazine supplied initially was converted to cyanuric acid and < 1% was converted to other s-triazines after prolonged incubation. Degradation proceeded via dechlorination and N-dealkylation. Atrazine was degraded until the concentration was circa 0.1 milligrams/liter. We obtained evidence showing that strain YAYA6 has specific uptake mechanisms for atrazine but less specific degradation mechanisms for s-triazines.
菌株YAYA6是从一个以莠去津作为唯一碳源生长并产生定量氯离子和硝酸盐的混合微生物群落中分离出来的。该菌株被鉴定为真假单胞菌群（RNA I组）的成员，并被命名为DMS 93-99。当莠去津作为唯一碳源和氮源时，生长产量为每摩尔莠去津产生80克（干重）细胞，细胞倍增时间约为11小时。在莠去津的初级降解过程中，约20%的[U-环14C]莠去津被矿化。莠去津从培养上清液中消失后，矿化作用仍在继续，直到矿化水平超过50%。在不同的实验条件下，经过长时间培养，最初供应的莠去津中有10%转化为氰尿酸，<1%转化为其他均三嗪。降解通过脱氯和N-脱烷基作用进行。莠去津降解至浓度约为0.1毫克/升。我们获得的证据表明，菌株YAYA6对莠去津具有特定的摄取机制，但对均三嗪的降解机制特异性较低。
