肉毒梭菌芽孢和营养细胞中的乳链菌肽抗性。
Nisin Resistance in Clostridium botulinum Spores and Vegetative Cells.
出版信息
Appl Environ Microbiol. 1997 Jul;63(7):2654-9. doi: 10.1128/aem.63.7.2654-2659.1997.
Abstract
The frequencies at which vegetative cells and spores of Clostridium botulinum strains 56A, 62A, 17409A, 25763A, 213B, B-aphis, and 169B formed colonies on agar media containing 0, 10(sup2), 10(sup3), and 10(sup4) IU of nisin per ml at 30(deg)C were determined. Strain 56A had the highest frequencies of nisin resistance, while strains 62A, 169B, and B-aphis had the lowest. For most strains, spores were more resistant than vegetative cells. One exposure to nisin was sufficient to generate stable nisin-resistant isolates in some strains. Stepwise exposure to increasing concentrations of nisin generated stable resistant isolates from all strains. Spores produced from nisin-resistant isolates maintained their nisin resistance. The frequency of spontaneous nisin resistance was reduced considerably by lowering the pH of the media and adding 3% NaCl. Nisin-resistant isolates of strains 56A and 169B also had increased resistance to pediocin PA1, bavaricin MN, plantaricin BN, and leuconocin S.
摘要
在 30°C 下，测定了含纳他霉素 0、10^2、10^3 和 10^4 IU/ml 的琼脂培养基中，凝结芽孢杆菌菌株 56A、62A、17409A、25763A、213B、B-aphis 和 169B 的营养细胞和孢子在琼脂培养基上形成菌落的频率。菌株 56A 具有最高的纳他霉素抗性频率，而菌株 62A、169B 和 B-aphis 具有最低的纳他霉素抗性频率。对于大多数菌株，孢子比营养细胞更具有抗性。在一些菌株中，一次暴露于纳他霉素足以产生稳定的纳他霉素抗性分离株。逐步暴露于递增浓度的纳他霉素可从所有菌株中产生稳定的抗性分离株。从纳他霉素抗性分离株产生的孢子保持其纳他霉素抗性。通过降低培养基的 pH 值并添加 3%NaCl，可大大降低自发纳他霉素抗性的频率。菌株 56A 和 169B 的纳他霉素抗性分离株对肠球菌素 PA1、巴氏杀菌素 MN、植物乳杆菌素 BN 和乳球菌素 S 的抗性也增加了。
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