Park Bong-Wook, Hah Young-Sool, Kim Deok Ryong, Kim Jong-Ryoul, Byun June-Ho
Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Research Institute of Life Science, Gyeongsang National University School of Medicine, 660-702 Jinju, Gyeongsangnam-do, South Korea.
Arch Oral Biol. 2007 Oct;52(10):983-9. doi: 10.1016/j.archoralbio.2007.04.007. Epub 2007 May 31.
Stem cells or osteogenic precursor cells isolated from bone marrow, trabecular tissues in bone, cartilage, muscle, and fat are the most suitable source for bone tissue engineering. In this study, we investigated the osteogenic phenotypes and mineralization of cultured human periosteal-derived cells obtained from mandibular periosteums. These periosteal-derived cells were positive for CD44, CD90, and CD166 antigens. They are successfully differentiated into osteoblasts in the medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. We observed that alkaline phosphatase (ALP) was largely expressed in the earlier stage of osteoblastic differentiation according to histochemical staining and RT-PCR analysis, whereas osteocalcin was dominantly expressed and secreted into the medium at the later stage. In addition, mineralized nodule formation has been observed by von Kossa staining in a time-dependent manner. These results suggest that periosteal-derived cell has the potential osteogenic activity and could be a good candidate for tissue engineering to restore the bony defects of the maxillofacial region.
从骨髓、骨小梁组织、软骨、肌肉和脂肪中分离出的干细胞或成骨前体细胞是骨组织工程最合适的细胞来源。在本研究中，我们调查了从下颌骨骨膜获得的培养人骨膜来源细胞的成骨表型和矿化情况。这些骨膜来源细胞对CD44、CD90和CD166抗原呈阳性反应。它们在含有地塞米松、抗坏血酸和β-甘油磷酸的培养基中成功分化为成骨细胞。根据组织化学染色和逆转录-聚合酶链反应分析，我们观察到碱性磷酸酶（ALP）在成骨细胞分化的早期大量表达，而骨钙素在后期主要表达并分泌到培养基中。此外，通过冯科萨染色观察到矿化结节形成具有时间依赖性。这些结果表明，骨膜来源细胞具有潜在的成骨活性，可能是用于修复颌面部骨缺损的组织工程的良好候选细胞。
