Kamau Sarah W, Hassa Paul O, Steitz Benedikt, Petri-Fink Alke, Hofmann Heinrich, Hofmann-Amtenbrink Margarethe, von Rechenberg Brigitte, Hottiger Michael O
Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
Nucleic Acids Res. 2006 Mar 15;34(5):e40. doi: 10.1093/nar/gkl035. Print 2006.
New approaches to increase the efficiency of non-viral gene delivery are still required. Here we report a simple approach that enhances gene delivery using permanent and pulsating magnetic fields. DNA plasmids and novel DNA fragments (PCR products) containing sequence encoding for green fluorescent protein were coupled to polyethylenimine coated superparamagnetic nanoparticles (SPIONs). The complexes were added to cells that were subsequently exposed to permanent and pulsating magnetic fields. Presence of these magnetic fields significantly increased the transfection efficiency 40 times more than in cells not exposed to the magnetic field. The transfection efficiency was highest when the nanoparticles were sedimented on the permanent magnet before the application of the pulsating field, both for small (50 nm) and large (200-250 nm) nanoparticles. The highly efficient gene transfer already within 5 min shows that this technique is a powerful tool for future in vivo studies, where rapid gene delivery is required before systemic clearance or filtration of the gene vectors occurs.
仍需要提高非病毒基因递送效率的新方法。在此，我们报告一种简单的方法，即利用永久磁场和脉动磁场增强基因递送。将含有绿色荧光蛋白编码序列的DNA质粒和新型DNA片段（PCR产物）与聚乙烯亚胺包被的超顺磁性纳米颗粒（SPIONs）偶联。将复合物添加到细胞中，随后使细胞暴露于永久磁场和脉动磁场。这些磁场的存在使转染效率显著提高，比未暴露于磁场的细胞高出40倍。对于小（50 nm）和大（200 - 250 nm）纳米颗粒，当在施加脉动磁场之前将纳米颗粒沉积在永久磁铁上时，转染效率最高。在5分钟内就实现了高效的基因转移，这表明该技术是未来体内研究的有力工具，因为在基因载体发生全身清除或过滤之前需要快速进行基因递送。
