Brown Dennis
Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, Mass 02114, USA.
Nephron Exp Nephrol. 2006;103(2):e55-61. doi: 10.1159/000090617. Epub 2006 Mar 10.
The need to understand complex intracellular trafficking mechanisms from both a basic and disease-oriented perspective has stimulated considerable interest in the development of real-time microscopy tools. Recent advances in instrumentation and the development of molecular bioprobes, such as green fluorescent protein and its derivatives, have opened up a new era in our ability to perform close to real-time imaging of cellular events with high spatial and temporal resolution, and with high sensitivity. This review briefly introduces and discusses some of the systems and methodologies that are available from several manufacturers, including laser scanning and spinning disk confocal microscopy, and total internal reflectance microscopy.
从基础和疾病导向的角度理解复杂的细胞内运输机制的需求，激发了人们对实时显微镜工具开发的浓厚兴趣。仪器设备的最新进展以及分子生物探针（如绿色荧光蛋白及其衍生物）的开发，开启了一个新时代，使我们能够以高空间和时间分辨率以及高灵敏度对细胞事件进行近乎实时的成像。本文简要介绍并讨论了几家制造商提供的一些系统和方法，包括激光扫描共聚焦显微镜、转盘共聚焦显微镜和全内反射显微镜。
