Wang Yucheng, Chu Yanguang, Liu Guifeng, Wang Myeong-Hyeon, Jiang Jing, Hou Yingjie, Qu Guanzheng, Yang Chuanping
Department of Forest Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040, China.
J Plant Physiol. 2007 Jan;164(1):78-89. doi: 10.1016/j.jplph.2005.12.006. Epub 2006 Mar 20.
Alkali grass (Puccinellia tenuiflora), a monocotyledonous halophyte, can serve as a model of salt tolerance in monocotyledon crops. To elucidate the molecular events associated with salt tolerance in alkali grass, we generated a directional cDNA library from leaves treated with the alkali salt, NaHCO3. Large-scale sequencing of the cDNA library identified 2942 ESTs representing 2366 non-redundant transcripts. These have been deposited in the dbEST division of GenBank. BLASTX evaluation indicated that 1274 of the ESTs were homologous to various known genes/proteins in a broad range of organisms, especially gramineae species. The other 1092 ESTs, which showed little if any homology to known sequences, were considered novel. Based on the encoded proteins, the 1274 identified ESTs fell into 12 functional categories, of which four were abundant, namely metabolism (18.84%), transcription (12.48%), unclassified (11.22%) and cell rescue/defense (9.66%). The 162 unique transcripts corresponding to possible salt-related genes were also identified. This study provides an overview of gene expression in NaHCO3-stressed alkali grass, as well as useful information for further investigation of salt resistance in plants.
碱茅（Puccinellia tenuiflora）是一种单子叶盐生植物，可作为单子叶作物耐盐性的模型。为了阐明碱茅中与耐盐性相关的分子事件，我们从用碱性盐NaHCO₃处理的叶片中构建了一个定向cDNA文库。对该cDNA文库进行大规模测序，鉴定出2942个EST，代表2366个非冗余转录本。这些已存入GenBank的dbEST部门。BLASTX评估表明，其中1274个EST与广泛生物体中的各种已知基因/蛋白质同源，尤其是禾本科物种。另外1092个EST与已知序列几乎没有同源性，被认为是新的。根据编码的蛋白质，鉴定出的1274个EST分为12个功能类别，其中四个类别丰富，即代谢（18.84%）、转录（12.48%）、未分类（11.22%）和细胞拯救/防御（9.66%）。还鉴定出了162个对应于可能的盐相关基因的独特转录本。本研究概述了NaHCO₃胁迫下碱茅中的基因表达情况，并为进一步研究植物的耐盐性提供了有用信息。
