De Tommasi N, Conti C, Stein M L, Pizza C
Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli, Italy.
Planta Med. 1991 Jun;57(3):250-3. doi: 10.1055/s-2006-960084.
A reinvestigation of the aerial parts of Calendula arvensis afforded, in addition to the oleanolic acid glycosides 1-4 (4), the new glycoside 5 whose structure was elucidated by spectral and chemical studies and determined as 3-O-(beta-D-galactopyranosyl-(1----3) [beta-D-glucopyranosyl-(1----4)]-beta-D-glucopyranosyl) oleanolic acid (28----1)-beta-D-glucopyranosyl ester. Furthermore, some antiviral tests were performed on glycosides 1-5 and on 5a, the hydrolysis product of 5, towards vesicular stomatitis virus (VSV) and rhinovirus (HRV) infection in cell cultures. An inhibitory effect against VSV multiplication was observed for all the compounds tested while HRV replication was significantly affected only by compound 3.
对田野金盏花地上部分的重新研究，除了得到齐墩果酸糖苷1 - 4（4）外，还得到了新的糖苷5。通过光谱和化学研究阐明了其结构，并确定为3 - O -（β - D - 吡喃半乳糖基 -（1→3）[β - D - 吡喃葡萄糖基 -（1→4）]-β - D - 吡喃葡萄糖基）齐墩果酸（28→1）-β - D - 吡喃葡萄糖基酯。此外，还对糖苷1 - 5以及5的水解产物5a进行了一些抗病毒测试，检测它们对细胞培养中水泡性口炎病毒（VSV）和鼻病毒（HRV）感染的影响。所有测试化合物对VSV增殖均有抑制作用，而只有化合物3对HRV复制有显著影响。
