经口鼻接种后犬类冠状病毒在犬体内的感染情况。
Canine coronavirus infection in the dog following oronasal inoculation.
作者信息
Tennant B J, Gaskell R M, Kelly D F, Carter S D, Gaskell C J
机构信息
Department of Veterinary Pathology, University of Liverpool.
出版信息
Res Vet Sci. 1991 Jul;51(1):11-8. doi: 10.1016/0034-5288(91)90023-h.
The pathogenesis of canine coronavirus (CCV) infection in 10-week-old puppies was studied up to 14 days after oronasal inoculation. Mild diarrhoea was seen from three to 11 days after inoculation, approximately coincident with faecal virus shedding. Virus was initially isolated from the tonsils on day 3, and then from both small and large intestinal tissues up to 14 days after inoculation. Virus was also isolated from liver and lung. Histological changes were not seen in any tissues, but CCV antigen was detected, using a peroxidase antiperoxidase staining technique, mainly in epithelium overlying gut-associated lymphoid tissue. Virus neutralising antibody was first detected on day 10. Specific anti-CCV IgM was first detected in plasma three days after inoculation and IgG on days 4 to 7. Small amounts of anti-CCV IgG, IgM and IgA were detected in duodenal secretion, but none in bile.
研究了10周龄幼犬经口鼻接种犬冠状病毒（CCV）后14天内的感染发病机制。接种后3至11天出现轻度腹泻，大致与粪便病毒排出时间一致。病毒最初于接种后第3天从扁桃体中分离出来，随后在接种后14天内从小肠和大肠组织中均分离出病毒。在肝脏和肺中也分离出了病毒。任何组织均未观察到组织学变化，但使用过氧化物酶抗过氧化物酶染色技术检测到CCV抗原，主要存在于肠道相关淋巴组织上方的上皮细胞中。病毒中和抗体于第10天首次检测到。特异性抗CCV IgM于接种后第3天在血浆中首次检测到，IgG于第4至7天检测到。十二指肠分泌物中检测到少量抗CCV IgG、IgM和IgA，但胆汁中未检测到。
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