Rimmelzwaan G F, Groen J, Egberink H, Borst G H, UytdeHaag F G, Osterhaus A D
Laboratory of Immunobiology, National Institute of Public Health and Environmental Protection, Bilthoven, Netherlands.
Vet Microbiol. 1991 Jan;26(1-2):25-40. doi: 10.1016/0378-1135(91)90039-i.
Complex trapping blocking (CTB) enzyme-linked immunosorbent assays (ELISAs) and indirect ELISAs for the detection of antibodies to canine parvovirus (CPV), canine coronavirus (CCV) and rotavirus in sera of dogs were established. Double antibody sandwich ELISAs for the detection of CPV-, CCV- and rotavirus antigens in fecal samples were also developed. Both the serological and antigen-detection ELISAs were used to screen samples from dogs in The Netherlands, with or without a history of acute diarrhea. It was shown that the results of the respective serological ELISAs correlated well and that CPV was the major cause of virus-induced acute diarrhea in dogs in The Netherlands.
建立了用于检测犬血清中犬细小病毒(CPV)、犬冠状病毒(CCV)和轮状病毒抗体的复合捕获阻断(CTB)酶联免疫吸附测定(ELISA)和间接ELISA。还开发了用于检测粪便样本中CPV、CCV和轮状病毒抗原的双抗体夹心ELISA。血清学ELISA和抗原检测ELISA均用于筛查荷兰有或无急性腹泻病史的犬的样本。结果表明,各自血清学ELISA的结果相关性良好,且CPV是荷兰犬病毒诱导的急性腹泻的主要原因。