用于检测犬冠状病毒及其抗体的酶联免疫吸附测定法。
Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs.
作者信息
Tuchiya K, Horimoto T, Azetaka M, Takahashi E, Konishi S
机构信息
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
出版信息
Vet Microbiol. 1991 Jan;26(1-2):41-51. doi: 10.1016/0378-1135(91)90040-m.
Abstract
Two methods of enzyme-linked immunosorbent assay (ELISA) were developed for the diagnosis of canine coronavirus (CCV) infection in dogs. One ELISA, in which CCV-infected CRFK cell lysate is used as antigen, is for the detection and titration of antibody against CCV, and the other ELISA uses the double antibody sandwich method for the detection of CCV antigen. The first ELISA procedure demonstrated antibody responses in dogs inoculated with CCV, as did the virus neutralization test; the second ELISA detected specific CCV antigen in feces and organ homogenates of inoculated dogs.
摘要
开发了两种酶联免疫吸附测定（ELISA）方法用于诊断犬冠状病毒（CCV）感染。一种ELISA以感染CCV的CRFK细胞裂解物作为抗原，用于检测和滴定抗CCV抗体，另一种ELISA采用双抗体夹心方法检测CCV抗原。第一种ELISA程序显示接种CCV的犬出现抗体反应，病毒中和试验也是如此；第二种ELISA在接种犬的粪便和器官匀浆中检测到特异性CCV抗原。
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本文引用的文献
1
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2
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J Biol Chem. 1951 Nov;193(1):265-75.
3
Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea.用于检测患有猪流行性腹泻的猪体内类冠状病毒及其抗体的酶联免疫吸附测定。
Vet Microbiol. 1982 Sep;7(4):295-306. doi: 10.1016/0378-1135(82)90009-8.
4
Epizootic of viral enteritis in dogs in Thailand.泰国犬类病毒性肠炎的流行
Am J Vet Res. 1982 Sep;43(9):1687-90.
5
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Arch Virol. 1980;66(3):215-26. doi: 10.1007/BF01314735.
6
Viruses and virus-like particles in the faeces of dogs with and without diarrhoea.患有腹泻和未患腹泻的犬类粪便中的病毒及病毒样颗粒。
Aust Vet J. 1984 Feb;61(2):33-8. doi: 10.1111/j.1751-0813.1984.tb07186.x.
7
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8
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