Kahle Kathrin, Kraus Michael, Scheppach Wolfgang, Ackermann Matthias, Ridder Friederike, Richling Elke
Department of Food Chemistry, University of Wuerzburg, Am Hubland, Germany.
Mol Nutr Food Res. 2006 Apr;50(4-5):418-23. doi: 10.1002/mnfr.200500211.
The aim of our studies was to determine the amount of polyphenols reaching the colon after oral intake of apple juice and blueberries. After a polyphenol-free diet healthy ileostomy volunteers consumed a polyphenol-rich cloudy apple juice while others consumed anthocyanin-rich blueberries. Ileostomy effluent was collected and polyphenols were identified using HPLC-DAD as well as HPLC-ESI-MS/MS; quantification was performed with HPLC-DAD. Most of the orally administered apple polyphenols were absorbed from or metabolized in the small intestine. Between 0 and 33% of the oral dose was recovered in the ileostomy bags with a maximum of excretion after 2 h. A higher amount of the blueberry anthocyanins under study (up to 85%, depending on the sugar moiety) were determined in the ileostomy bags and therefore would reach the colon under physiological circumstances. Such structure-related availability has to be considered when polyphenols are used in model systems to study potential preventive effects in colorectal diseases.
我们研究的目的是确定口服苹果汁和蓝莓后到达结肠的多酚量。在无多酚饮食后，健康的回肠造口术志愿者饮用富含多酚的混浊苹果汁，而其他志愿者则食用富含花青素的蓝莓。收集回肠造口术流出物，并使用HPLC-DAD以及HPLC-ESI-MS/MS鉴定多酚；使用HPLC-DAD进行定量。口服的苹果多酚大部分在小肠中被吸收或代谢。口服剂量的0%至33%在回肠造口袋中回收，2小时后排泄量最大。在回肠造口袋中测定了更高量的所研究的蓝莓花青素（高达85%，取决于糖部分），因此在生理情况下会到达结肠。当在模型系统中使用多酚来研究对结直肠癌的潜在预防作用时，必须考虑这种与结构相关的可利用性。
