病毒信使核糖核酸在体外的甲基化依赖性翻译
Methylation-dependent translation of viral messenger RNAs in vitro.
作者信息
Both G W, Banerjee A K, Shatkin A J
出版信息
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1189-93. doi: 10.1073/pnas.72.3.1189.
Abstract
Methylated reovirus and vesicular stomatitis virus mRNAs, synthesized in vitro in the presence of S-adenosylmethionine by the virion-associated polymerases (RNA nucleotidyltransferases, EC 2.7.7.6), stimulate protein synthesis by wehat germ extracts to a greater extent than unmethylated mRNAs. Addition of S-adenosylmethionine to a cell-free extract programmed with unmethylated mRNA stimulates protein synthesis and results in methylation of the mRNA. An inhibitor of mRNA methylation. S-adenosylhomocysteine, blocks translation of unmethylated, but not of methylated, mRNAs. Aurintricarboxylic acid, which inhibits polypepetide chain initiation, also prevents mRNA methylation by wheat germ extracts. In contrast, sparsomycin, which inhibits polypeptide chain elongation, does not reduce mRNA methylation. The results indicate that methylation of viral mRNA is required for translation in vitro and suggest that mRNA methylation occurs at the initiation step of protein synthesis.
摘要
在S-腺苷甲硫氨酸存在的情况下，由病毒体相关聚合酶（RNA核苷酸转移酶，EC 2.7.7.6）在体外合成的甲基化呼肠孤病毒和水疱性口炎病毒mRNA，比未甲基化的mRNA更能刺激小麦胚芽提取物的蛋白质合成。向用未甲基化mRNA编程的无细胞提取物中添加S-腺苷甲硫氨酸可刺激蛋白质合成，并导致mRNA甲基化。mRNA甲基化抑制剂S-腺苷同型半胱氨酸可阻断未甲基化mRNA的翻译，但不影响甲基化mRNA的翻译。抑制多肽链起始的金精三羧酸也可阻止小麦胚芽提取物对mRNA的甲基化。相反，抑制多肽链延伸的稀疏霉素不会降低mRNA甲基化。结果表明，病毒mRNA的甲基化是体外翻译所必需的，并且表明mRNA甲基化发生在蛋白质合成的起始步骤。
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