真核生物信使核糖核酸中的5'-末端7-甲基鸟苷是翻译所必需的。
5'-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation.
作者信息
Muthukrishnan S, Both G W, Furuichi Y, Shatkin A J
出版信息
Nature. 1975 May 1;255(5503):33-7. doi: 10.1038/255033a0.
DOI:10.1038/255033a0
PMID:165427
Abstract
Unmethylated reovirus and VSV mRNAs are specifically methylated to form 5'-terminal structures of the type, m-7-G(5')ppp(5')N by protein synthesising extracts prepared from wheat germ and mouse L cells. Reticulocyte mRNA also contains 5'-terminal m-7-G. MRNAs having 5'-terminal m-7-G stimulate protein synthesis in vitro. Removal of m-7-G by beta-elimination abolishes translation of the mRNAs.
摘要
未甲基化的呼肠孤病毒和水泡性口炎病毒信使核糖核酸（mRNA）通过从小麦胚芽和小鼠L细胞制备的蛋白质合成提取物被特异性甲基化，形成m-7-G(5')ppp(5')N类型的5'-末端结构。网织红细胞mRNA也含有5'-末端m-7-G。具有5'-末端m-7-G的mRNA在体外刺激蛋白质合成。通过β-消除去除m-7-G会使mRNA的翻译终止。
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