Nuss D L, Oppermann H, Koch G
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1258-62. doi: 10.1073/pnas.72.4.1258.
Poliovirus mRNA and mRNA transcribed from vesicular stomatitis virus and reovirus genomes efficiently direct protein synthesis in vivo under experimental conditions where the initiation of host protein synthesis is selectively blocked. The selective blockage of host peptide chain initiation after exposure to hypertonic medium indicates that the translation of viral mRNA is more efficiently initiated than is the translation of host mRNA. It further suggests that virus directed suppression of host protein synthesis could proceed by a mechanism involving a nonspecific decrease in the rate of peptide chain initiation. Exposure of infected cells to hypertonic medium provides a unique tool with which to study early events in the infectious cycle by permitting the efficient unmasking of virus-specific poly-peptide synthesis.
在实验条件下，当宿主蛋白质合成的起始被选择性阻断时，脊髓灰质炎病毒mRNA以及从水疱性口炎病毒和呼肠孤病毒基因组转录而来的mRNA能够在体内有效地指导蛋白质合成。暴露于高渗培养基后宿主肽链起始的选择性阻断表明，病毒mRNA的翻译比宿主mRNA的翻译更有效地起始。这进一步表明，病毒介导的对宿主蛋白质合成的抑制可能通过一种涉及肽链起始速率非特异性降低的机制进行。将感染细胞暴露于高渗培养基提供了一种独特的工具，通过允许有效地揭示病毒特异性多肽合成来研究感染周期中的早期事件。
