Rose J K, Trachsel H, Leong K, Baltimore D
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2732-6. doi: 10.1073/pnas.75.6.2732.
Translation of vesicular stomatitis virus (VSV) mRNA, like host mRNA translation, is inhibited in cells infected with poliovirus. To study the mechanism of poliovirus-induced inhibition of protein synthesis, we prepared extracts from poliovirus-infected and uninfected HeLa cells. Poliovirus mRNA was translated in lysates from both infected and uninfected cells, while VSV mRNA was translated only in the lysate from uninfected cells. Addition of purified translation initiation factors to the extract from infected cells showed that one factor, eIF-4B, could restore VSV mRNA translation in the infected lysate, but did not increase poliovirus mRNA translation. Further experiments involving translation of VSV mRNA in mixed extracts from poliovirus-infected and uninfected cells showed (i) that there was not an excess of an inhibitor of VSV mRNA translation in the infected lysate, but (ii) that an acitivity that caused a slow inactivation of eIF-4B was present in the infected lysate. Inactivation of eIF-4B appears to be the mechanism by which poliovirus infection causes a selective inhibition of translation.
水泡性口炎病毒（VSV）mRNA的翻译，如同宿主mRNA的翻译一样，在感染脊髓灰质炎病毒的细胞中受到抑制。为了研究脊髓灰质炎病毒诱导的蛋白质合成抑制机制，我们从感染和未感染脊髓灰质炎病毒的HeLa细胞中制备了提取物。脊髓灰质炎病毒mRNA在感染和未感染细胞的裂解物中均能翻译，而VSV mRNA仅在未感染细胞的裂解物中翻译。向感染细胞的提取物中添加纯化的翻译起始因子表明，一种因子eIF-4B能够恢复感染裂解物中VSV mRNA的翻译，但不会增加脊髓灰质炎病毒mRNA的翻译。进一步涉及在脊髓灰质炎病毒感染和未感染细胞的混合提取物中翻译VSV mRNA的实验表明：（i）感染裂解物中不存在过量的VSV mRNA翻译抑制剂；（ii）感染裂解物中存在一种导致eIF-4B缓慢失活的活性物质。eIF-4B的失活似乎是脊髓灰质炎病毒感染导致翻译选择性抑制的机制。
