Morrison T, Stampfer M, Baltimore D, Lodish H F
J Virol. 1974 Jan;13(1):62-72. doi: 10.1128/JVI.13.1.62-72.1974.
RNA was isolated from polyribosomes of vesicular stomatitis virus (VSV)-infected cells and tested for its ability to direct protein synthesis in extracts of animal and plant cells. In cell-free, non-preincubated extracts of rabbit reticulocytes, the 28S VSV RNA stimulated synthesis of a protein the size of the vesicular stomatitis virus L protein whereas the 13 to 15S RNA directed synthesis of the VSV M, N, NS, and possibly G proteins. In wheat germ extracts, 13 to 15S RNA also directed synthesis of the N, NS, M, and possibly G proteins. Analysis of extracts labeled with formyl [(35)S]methionine showed that the 28S RNA directed the initiation of synthesis of one protein, whereas the 13 to 15S RNA directed initiation of at least four proteins. It is concluded that the 28S RNA encodes only the L protein, whereas the 13 to 15S RNA is a mixture of species, presumably monocistronic, which code for the four other known vesicular stomatitis virus proteins.
从感染水泡性口炎病毒（VSV）的细胞的多核糖体中分离出RNA，并检测其在动物和植物细胞提取物中指导蛋白质合成的能力。在兔网织红细胞的无细胞、未预孵育提取物中，28S VSV RNA刺激合成一种大小与水泡性口炎病毒L蛋白相同的蛋白质，而13至15S RNA指导VSV M、N、NS以及可能的G蛋白的合成。在小麦胚芽提取物中，13至15S RNA也指导N、NS、M以及可能的G蛋白的合成。对用甲酰基[（35）S]甲硫氨酸标记的提取物的分析表明，28S RNA指导一种蛋白质合成的起始，而13至15S RNA指导至少四种蛋白质合成的起始。得出的结论是，28S RNA仅编码L蛋白，而13至15S RNA是多种RNA的混合物，推测为单顺反子，它们编码其他四种已知的水泡性口炎病毒蛋白。
