检测胎牛血清中牛病毒性腹泻病毒及抗牛病毒性腹泻病毒抗体的污染方法和污染频率。

Methods for detection and frequency of contamination of fetal calf serum with bovine viral diarrhea virus and antibodies against bovine viral diarrhea virus.

作者信息

Bolin S R, Matthews P J, Ridpath J F

机构信息

Virology Cattle Research Unit, USDA, Agricultural Research Service, Ames, IA 50010.

出版信息

J Vet Diagn Invest. 1991 Jul;3(3):199-203. doi: 10.1177/104063879100300302.

DOI:10.1177/104063879100300302

PMID:1655059

Abstract

Methods used by the National Animal Disease Center to test fetal calf serum for contamination with bovine viral diarrhea virus (BVDV) and antibodies against BVDV are described. Using those methods, virus was isolated from 332 of 1,608 (20.6%) lots of raw fetal calf serum obtained specifically for the Center and 93 of 190 (49%) lots of commercially available fetal calf serum. Virus neutralization and immunoperoxidase staining tests were used to detect antibodies against BVDV in 224 of the 1,608 (13.9%) lots of raw fetal calf serum. Both BVDV and antibodies against BVDV were detected in 50 lots of raw serum. The molecular specificity of antibodies against BVDV was determined by radioimmunoprecipitation. Lots of fetal calf serum that contained BVDV-specific antibodies that did not neutralize virus were identified.

摘要

本文描述了国家动物疾病中心检测胎牛血清中牛病毒性腹泻病毒（BVDV）污染及抗BVDV抗体的方法。使用这些方法，从专门为该中心获取的1608份生胎牛血清样本中的332份（20.6%）以及190份市售胎牛血清样本中的93份（49%）中分离出了病毒。采用病毒中和试验和免疫过氧化物酶染色试验，在1608份生胎牛血清样本中的224份（13.9%）中检测到了抗BVDV抗体。在50份生血清中同时检测到了BVDV和抗BVDV抗体。通过放射免疫沉淀法确定了抗BVDV抗体的分子特异性。鉴定出了含有未中和病毒的BVDV特异性抗体的胎牛血清样本。