Bolin S R, Ridpath J F
Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
J Vet Diagn Invest. 1998 Apr;10(2):135-9. doi: 10.1177/104063879801000203.
One thousand lots of pooled fetal bovine serum (FBS) were tested for contamination with bovine viral diarrhea virus (BVDV) and/or for contamination with neutralizing antibody against BVDV. Noncytopathic or cytopathic BVDV was isolated from 203 lots of FBS. Analysis of the viral isolates identified 115 type 1 and 65 type 2 BVDV isolates. An additional 23 virus isolates were mixtures of > or = 2 BVDV isolates and were not classified to viral genotype. Further characterization of the type 1 viruses identified 51 subgenotype 1a and 64 subgenotype 1b BVDV isolates. Viral neutralizing antibody was detected in 113 lots of FBS. Differential viral neutralization indicated that type 1 BVDV induced the antibody detected in 48 lots of FBS and type 2 BVDV induced the antibody detected in 16 lots of FBS.
对1000批混合胎牛血清（FBS）进行了牛病毒性腹泻病毒（BVDV）污染检测和/或抗BVDV中和抗体污染检测。从203批FBS中分离出非细胞病变型或细胞病变型BVDV。对病毒分离株的分析确定了115株1型和65株2型BVDV分离株。另外23株病毒分离株是≥2种BVDV分离株的混合物，未分类到病毒基因型。对1型病毒的进一步鉴定确定了51株1a亚型和64株1b亚型BVDV分离株。在113批FBS中检测到病毒中和抗体。差异病毒中和表明，1型BVDV诱导了在48批FBS中检测到的抗体，2型BVDV诱导了在16批FBS中检测到的抗体。
