Stricker Sigmar, Brieske Norbert, Haupt Julia, Mundlos Stefan
Max Planck Institute for Molecular Genetics, Berlin, Germany.
Gene Expr Patterns. 2006 Oct;6(8):826-34. doi: 10.1016/j.modgep.2006.02.003. Epub 2006 Mar 22.
Odd-skipped genes encode zinc-finger transcription factors with widespread roles in embryonic development. In Drosophila, odd-skipped acts as a pair-rule gene, while its orthologous gene in Caenorhabditis elegans is involved in gut development. In mammals two paralogs exist, Osr1 and Osr2, with functions described in heart and urogenital, and in secondary palate development, respectively. As the chicken embryo is a widely used system for analysing gene function in vivo, we determined the expression pattern of the two chicken orthologues, cOsr1 and cOsr2, during embryonic development. We demonstrate expression of both genes in a variety of organs and structures, such as kidney, eye, branchial arches and dermis. Both genes show a highly dynamic expression pattern with partially overlapping, but mostly distinct domains of expression. Special emphasis in this study was laid on the investigation of cOsr1 and cOsr2 in limb development, where we compared their expression pattern with the expression of Osr1 and Osr2 in the mouse.
奇跳基因编码锌指转录因子，在胚胎发育中具有广泛作用。在果蝇中，奇跳基因作为一种体节极性基因发挥作用，而其在秀丽隐杆线虫中的直系同源基因则参与肠道发育。在哺乳动物中存在两个旁系同源基因，即Osr1和Osr2，它们分别在心脏和泌尿生殖系统以及次生腭发育中发挥作用。由于鸡胚是用于体内分析基因功能的广泛使用的系统，我们确定了两个鸡直系同源基因cOsr1和cOsr2在胚胎发育过程中的表达模式。我们证明这两个基因在多种器官和结构中表达，如肾脏、眼睛、鳃弓和真皮。这两个基因都显示出高度动态的表达模式，其表达域部分重叠，但大多不同。本研究特别强调了对cOsr1和cOsr2在肢体发育中的研究，我们将它们的表达模式与小鼠中Osr1和Osr2的表达进行了比较。
