Institute of Hygiene and Microbiology, University of Würzburg, Germany.
Infect Immun. 1970 Mar;1(3):251-8. doi: 10.1128/iai.1.3.251-258.1970.
We carried out a study on the adjuvant effect of Bordetella pertussis vaccine on the primary and secondary immune responses of the mouse to sheep erythrocytes, quantitating antibody-producing spleen cells and serum antibody. The simultaneous injection of sheep erythrocytes and B. pertussis, when compared to immunization with sheep red blood cells alone, resulted in an increased and prolonged multiplication of antibody-forming spleen cells. The adjuvant effect was also documented by increased production of serum hemolysins and agglutinins. Further, B. pertussis enhanced the priming effect of the antigen for the secondary response. However, when the bacterial adjuvant was given together with a second antigenic stimulus 41 days after the primary immunization, the peak values of direct and indirect plaque-forming spleen cells did not differ from the corresponding control animals further inoculated with sheep erythrocytes alone. Nonetheless, the influence of the bacterial adjuvant was still expressed by the delayed decrease of the numbers of plaque-forming spleen cells. On the basis of the X-Y-Z scheme it is suggested that B. pertussis cells as adjuvant enhance the multiplication of antigen-sensitive X cells or affect the initial stages of differentiation of these cells. This effect of the pertussis vaccine can be distinguished from a general proliferative action on other cells.
我们进行了一项关于百日咳杆菌疫苗对绵羊红细胞初次和再次免疫小鼠的辅助作用的研究，定量检测了产生抗体的脾细胞和血清抗体。与单独免疫绵羊红细胞相比，同时注射绵羊红细胞和百日咳杆菌导致抗体形成脾细胞的增殖增加和持续时间延长。通过增加血清溶血素和凝集素的产生，也证明了佐剂的作用。此外，百日咳杆菌增强了抗原对二次反应的引发效应。然而，当在初次免疫后 41 天给予细菌佐剂和第二种抗原刺激时，直接和间接溶血空斑形成脾细胞的峰值与进一步单独接种绵羊红细胞的相应对照动物没有差异。尽管如此，细菌佐剂的影响仍然表现为溶血空斑形成脾细胞数量的延迟减少。根据 X-Y-Z 方案，百日咳杆菌细胞作为佐剂增强了抗原敏感 X 细胞的增殖或影响这些细胞的初始分化阶段。这种百日咳疫苗的作用可以与对其他细胞的一般增殖作用区分开来。
