Department of Pathology, Baptist Memorial Hospital, and Department of Microbiology, University of Tennessee Medical Units, Memphis, Tennessee 38103.
Infect Immun. 1971 Jan;3(1):127-32. doi: 10.1128/iai.3.1.127-132.1971.
Successful growth of Mycobacterium lepraemurium has been achieved by use of a specialized diffusion chamber technique. The cell-impermeable porous chambers were maintained in animals for periods up to 50 days with and without macrophages and LM cells. A generation time of 6 to 8 days was found for the acid-fast bacilli in chambers containing macrophages when maintained in the mouse. Also, cell-free chambers maintained in the mouse gave a generation time of 11 days for M. lepraemurium. There was no doubt that chambers maintained in a susceptible host provided greater yields of bacilli than chambers maintained in a nonsusceptible host such as the guinea pig. In fact, better yields were obtained when the chambers were maintained in monolayer petri plate cultures of mouse peritoneal macrophages than when held in the guinea pig. The most pertinent observation was that living cells are not essential for growth of M. lepraemurium, and the results suggest that multiplication can occur in a cell-free environment within a susceptible host. These studies give evidence that the use of porous chambers has promising possibilities for further investigations on the cultivation of other fastidious mycobacteria.
成功地使麻风分枝杆菌生长的方法是采用特殊的扩散室技术。将这种不能穿透细胞的多孔室保存在动物体内，不加或加巨噬细胞和淋巴细胞，时间长达 50 天。在含巨噬细胞的室内，当保持在鼠体内时，麻风分枝杆菌的代时为 6 至 8 天。同样，保存在鼠体内的无细胞室中麻风分枝杆菌的代时为 11 天。毫无疑问，在易感宿主中保持的室内比在不易感宿主如豚鼠中保持的室内能产生更多的杆菌。事实上，当室保持在鼠腹膜巨噬细胞单层 petri 板培养物中时，比保持在豚鼠中时，能得到更好的产量。最值得注意的观察结果是，活细胞不是麻风分枝杆菌生长所必需的，结果表明，在易感宿主的无细胞环境中可以发生增殖。这些研究证明，多孔室的使用为进一步研究其他苛养分枝杆菌的培养提供了有希望的可能性。
