Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455.
Infect Immun. 1971 Jun;3(6):723-6. doi: 10.1128/iai.3.6.723-726.1971.
The fatty acid requirements of two avirulent treponemes were investigated by using a "lipid-poor" albumin-thioglycolate medium. The Kazan 5 and the Reiter stains of Treponema pallidum required a pair of fatty acids for growth. One member of the pair was saturated and the other was unsaturated. The saturated fatty acids could contain either an odd or even number of carbon atoms, but a chain length of at least 14 carbon atoms was necessary. Unsaturated fatty acids with one, two, or three double bonds were satisfactory if they contained 15 or more carbon atoms. The pair of fatty acids could be replaced with a single 18-carbon monounsaturated fatty acid if it was in the trans configuration rather than the naturally occurring cis form.
用“贫脂”白蛋白-巯基醋酸盐培养基研究了两种无毒密螺旋体的脂肪酸需要。苍白密螺旋体的卡赞 5 号和赖特染色株需要一对脂肪酸才能生长。这对脂肪酸中的一个成员是饱和的,另一个是不饱和的。饱和脂肪酸可以含有奇数或偶数个碳原子,但至少需要 14 个碳原子的链长。如果含有 15 个或更多碳原子,则具有一个、两个或三个双键的不饱和脂肪酸是令人满意的。如果这对脂肪酸是反式构型而不是天然的顺式形式,那么可以用单个 18 碳单不饱和脂肪酸代替。