Caldwell H D, Kuo C C, Kenny G E
J Immunol. 1975 Oct;115(4):969-75.
Two-dimensional immunoelectrophoresis was utilized to study precipitins in hyperimmune rabbit serum made against chlamydiae and from patients with chlamydial infections. An antigen of Triton X-100-solubilized L2/434/Bu organisms with an electrophoretic mobility of 0.65 relative to bovine serum albumin at pH 8.6 was excised from the agarose gel of electrophorograms as antigen-antibody complexes and used to immunize rabbits. A monospecific antiserum to antigen 0.65 was obtained that reacted with Trachoma-LGV strains L2/434/Bu, B/TW-5/OT, and K/UW-31/Cx, but not with the mouse pneumonitis (Nigg) strain or the psittacosis strain meningopneumonitis (Cal-10). The Trachoma-LGV specificity of antigen 0.65 was further shown by indirect immunofluorescence straining with the monospecific antiserum of chlamydial inclusions in infected HeLa cells. Precipitins with a specificity for antigen 0.65 were indentified in 15 of 18 sera from patients with diagnosed Chlamydia trachomatis infections LGV, trachoma, nongonococcal urethritis, and nongonococcal cervicitis by using monospecific antiserum to antigen 0.65 in the peak suppression test. Thus, antigen 0.65 appears to be a Trachoma-LGV-specific antigen that has considerable promise for serodiagnosis.
利用二维免疫电泳研究针对衣原体的超免疫兔血清以及衣原体感染患者血清中的沉淀素。从电泳图谱的琼脂糖凝胶中切下一种Triton X - 100溶解的L2/434/Bu菌株抗原，在pH 8.6条件下其相对于牛血清白蛋白的电泳迁移率为0.65，作为抗原 - 抗体复合物用于免疫兔子。获得了一种针对抗原0.65的单特异性抗血清，它与沙眼 - 淋巴肉芽肿性尿道炎（LGV）菌株L2/434/Bu、B/TW - 5/OT和K/UW - 31/Cx发生反应，但不与小鼠肺炎（Nigg）菌株或鹦鹉热菌株脑膜肺炎（Cal - 10）发生反应。用该单特异性抗血清对感染的HeLa细胞中的衣原体包涵体进行间接免疫荧光染色，进一步证明了抗原0.65的沙眼 - LGV特异性。在确诊为沙眼衣原体感染（LGV、沙眼、非淋菌性尿道炎和非淋菌性宫颈炎）的18例患者的血清中，有15例通过在峰抑制试验中使用针对抗原0.65的单特异性抗血清鉴定出了对抗原0.65具有特异性的沉淀素。因此，抗原0.65似乎是一种沙眼 - LGV特异性抗原，在血清学诊断方面具有很大的前景。
