Levy R, Segal E, Eylan E
Infect Immun. 1981 Mar;31(3):874-8. doi: 10.1128/iai.31.3.874-878.1981.
Candida albicans ribosomes were prepared from mechanically disrupted cells through differential centrifugation and purification in a sucrose-ammonium sulfate solution. The ribosomes were analyzed chemically and physically and exhibited characteristics of eucaryotic ribosomes (78S). ICR female mice were immunized with two subcutaneous inoculations, 2 weeks apart, of 100 microgram of ribosomes (expressed as ribosomal protein). Immunized mice were challenged either intraperitoneally or intravenously with a lethal dose of live C. albicans cells. The 31-day survival rate of immunized mice challenged intraperitoneally was 64% (mean value) versus 27% in controls; in intravenously challenged mice the survival rate of the immunized animals was about 60%, with no survivors among the controls. In intravenously challenged mice, incomplete Freund adjuvant enhanced the protection elicited by the ribosomes. Protection by ribosomal immunization was obtained against challenge doses causing chronic and acute infection.
白色念珠菌核糖体是通过在蔗糖 - 硫酸铵溶液中对机械破碎的细胞进行差速离心和纯化制备的。对这些核糖体进行了化学和物理分析，结果显示其具有真核核糖体（78S）的特征。用100微克核糖体（以核糖体蛋白表示）对ICR雌性小鼠进行皮下接种免疫，间隔2周进行两次接种。免疫后的小鼠通过腹腔注射或静脉注射致死剂量的活白色念珠菌细胞进行攻毒。腹腔注射攻毒的免疫小鼠31天存活率为64%（平均值），而对照组为27%；静脉注射攻毒的小鼠中，免疫动物的存活率约为60%，对照组无存活者。在静脉注射攻毒的小鼠中，不完全弗氏佐剂增强了核糖体引发的保护作用。通过核糖体免疫可获得针对引起慢性和急性感染的攻毒剂量的保护。
