Leon L L, Leon W, Chaves L, Costa S C, Cruz M Q, Brascher H M, Lima A O
Infect Immun. 1980 Jan;27(1):38-43. doi: 10.1128/iai.27.1.38-43.1980.
Studies were carried out with a polyribosomal fraction isolated from Trypanosoma cruzi Y epimastigotes, with the intention to determine both its immunogenic activity and the degree of protection it could induce against experimental T. cruzi infection. This fraction was assayed in four groups of mice by using different schedules of vaccination and varying the dose, intervals, and route of administration. Seven days after the last dose, the animals were sacrificed for immunological studies or subjected to challenge with T. cruzi trypomastigotes. The results obtained in all schedules showed that our polyribosomal fraction only induced a weak antibody response, but was capable of evoking an expressive cellular response. It was also shown that this fraction has the capacity of inducing a high degree of protection against T. cruzi infection, as determined by the decrease of parasitemia and the prolonged survival time of immunized animals.
对从克氏锥虫Y型上鞭毛体分离出的多核糖体组分进行了研究，目的是确定其免疫原活性以及它对实验性克氏锥虫感染可诱导的保护程度。通过使用不同的疫苗接种方案以及改变剂量、间隔时间和给药途径，在四组小鼠中对该组分进行了检测。最后一剂接种七天后，处死动物进行免疫学研究，或用克氏锥虫锥鞭毛体进行攻击。在所有方案中获得的结果表明，我们的多核糖体组分仅诱导了微弱的抗体反应，但能够引发明显的细胞反应。还表明，通过免疫动物的寄生虫血症降低和存活时间延长来确定，该组分具有诱导对克氏锥虫感染高度保护的能力。
