感染腺病毒12以及感染腺病毒12加猴病毒40的绿猴肾细胞的免疫荧光。
Immunofluorescence of green monkey kidney cells infected with adenovirus 12 and with adenovirus 12 plus simian virus 40.
作者信息
Malmgren R A, Rabson A S, Carney P G, Paul F J
机构信息
Pathologic Anatomy Branch and Laboratory of Pathology, National Cancer Institute, U.S. Public Health Service, Bethesda, Maryland.
出版信息
J Bacteriol. 1966 Jan;91(1):262-5. doi: 10.1128/jb.91.1.262-265.1966.
Malmgren, Richard A. (National Cancer Institute; Bethesda, Md.), Alan S. Rabson, Paula G. Carney, and Frances J. Paul. Immunofluorescence of green monkey kidney cells infected with adenovirus 12 and with adenovirus 12 plus simian virus 40. J. Bacteriol. 91:262-265. 1966.-Immunofluorescence studies of the viral antigens and tumor (T) antigens of adenovirus 12 and simian virus 40 (SV40) in green monkey kidney (GMK) cells infected with adenovirus 12 alone or in combination with the SV40 virus showed that the adenovirus 12 viral antigen was produced in detectable amounts only in the cells infected with both viruses. The adenovirus 12 T antigen, on the other hand, was formed in the GMK cells infected with the adenovirus 12 only. This antigen was formed as early as 18 hr after viral infection, and persisted for at least 48 hr after virus infection. There was a correlation between the appearance of the immunofluorescent T antigen in the nucleus and the electron microscope appearance of "nuclear stippling," which developed in the nuclei of GMK cells after infection with adenovirus 12 only, as well as after infection with both viruses.
马尔姆格伦，理查德·A.（美国国立癌症研究所；马里兰州贝塞斯达），艾伦·S.拉布森，宝拉·G.卡尼，以及弗朗西斯·J.保罗。感染腺病毒12以及腺病毒12加猴病毒40的绿猴肾细胞的免疫荧光。《细菌学杂志》91:262 - 265。1966年。——对单独感染腺病毒12或与猴病毒40（SV40）联合感染的绿猴肾（GMK）细胞中腺病毒12和猴病毒40（SV40）的病毒抗原及肿瘤（T）抗原进行的免疫荧光研究表明，仅在同时感染两种病毒的细胞中才能检测到腺病毒12病毒抗原的产生。另一方面，腺病毒12 T抗原仅在感染腺病毒12的GMK细胞中形成。这种抗原在病毒感染后18小时就开始形成，并在病毒感染后至少持续48小时。细胞核中免疫荧光T抗原的出现与“核点彩”的电子显微镜表现之间存在相关性，“核点彩”仅在单独感染腺病毒12的GMK细胞以及同时感染两种病毒的细胞的细胞核中出现。
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