Eron L
J Virol. 1975 May;15(5):1256-61. doi: 10.1128/JVI.15.5.1256-1261.1975.
Infection of the continuous simian cell lines CV-1 and BSC-1 with human adenovirus type 2 (Ad2) is abortive. However, the restriction of Ad2 reproduction in these cells can be overcome by increasing the Ad2 infectious dose or by coinfection with simian virus 40. Vero, another established simian cell line free of detectable endogenous simian virus 40 DNA, is not restricted in its ability to promote Ad2 growth even at low input multiplicities of Ad2 and in the absence of SV40 helper. The amount of structural Ad2 proteins in total cell extracts of enhanced BSC-1 cells is at least two orders of magnitude higher than that of unenhanced cells. In contrast, comparable quantities of Ad2 mRNA specifying these proteins are found in both the enhanced and the unenhanced cell. Both sets of mRNA can be translated in a cell-free system with equal efficiency.
用2型人腺病毒（Ad2）感染连续的猴细胞系CV-1和BSC-1是流产性的。然而，通过增加Ad2感染剂量或与猴病毒40共感染，可以克服Ad2在这些细胞中的繁殖限制。Vero是另一种已建立的猴细胞系，未检测到内源性猴病毒40 DNA，即使在低Ad2输入复数且不存在SV40辅助病毒的情况下，其促进Ad2生长的能力也不受限制。增强的BSC-1细胞总细胞提取物中Ad2结构蛋白的量比未增强的细胞至少高两个数量级。相比之下，在增强细胞和未增强细胞中都发现了指定这些蛋白的相当数量的Ad2 mRNA。两组mRNA都可以在无细胞系统中以相同效率进行翻译。
