Rapp F, Feldman L A, Mandel M
J Bacteriol. 1966 Oct;92(4):931-6. doi: 10.1128/jb.92.4.931-936.1966.
Rapp, Fred (Baylor University College of Medicine, Houston, Tex.), Lawrence A. Feldman, and Manley Mandel. Synthesis of virus deoxyribonucleic acid during abortive infection of simian cells by human adenoviruses. J. Bacteriol. 92:931-936. 1966.-Inoculation of green monkey kidney cells (GMK) with adenovirus types 2 or 12, under conditions where neither infectious virus was synthesized, resulted in an increase in the uptake of H(3)-thymidine into deoxyribonucleic acid (DNA). Extraction of the DNA from infected cells, followed by identification by isopycnic analysis in CsCl gradients, revealed the presence of virus DNA. Cells infected with adenovirus type 2 yielded DNA giving bands with peak densities of 1.699 g/ml [GMK DNA with 40 moles% guanine + cytosine (GC)] and 1.714 g/ml (adenovirus type 2 DNA with 55 moles% GC). Cells infected with adenovirus type 12 also yielded the GMK DNA and a band at 1.706 g/ml (adenovirus type 12 DNA with 47 moles% GC). The rate of synthesis of adenovirus type 2 DNA in KB cells (productive cycle) and in GMK cells infected only with adenovirus (nonproductive cycle) or with adenovirus and simian virus 40 (adeno-productive cycle) was not significantly different.
拉普，弗雷德（得克萨斯州休斯敦贝勒医学院）、劳伦斯·A·费尔德曼和曼利·曼德尔。人腺病毒对猿猴细胞进行流产感染期间病毒脱氧核糖核酸的合成。《细菌学杂志》92:931 - 936。1966年。——在既不合成感染性病毒的条件下，用2型或12型腺病毒接种绿猴肾细胞（GMK），导致H(3)-胸苷掺入脱氧核糖核酸（DNA）的量增加。从感染细胞中提取DNA，随后通过在CsCl梯度中的等密度分析进行鉴定，结果显示存在病毒DNA。感染2型腺病毒的细胞产生的DNA在密度峰值为1.699克/毫升[含40摩尔%鸟嘌呤 + 胞嘧啶（GC）的GMK DNA]和1.714克/毫升（含55摩尔%GC的2型腺病毒DNA）处出现条带。感染12型腺病毒的细胞也产生了GMK DNA以及在1.706克/毫升处的一条带（含47摩尔%GC的12型腺病毒DNA）。2型腺病毒DNA在KB细胞（生产性周期）以及仅感染腺病毒的GMK细胞（非生产性周期）或同时感染腺病毒和猿猴病毒40的GMK细胞（腺病毒 - 生产性周期）中的合成速率没有显著差异。
