大肠杆菌吡哆醛营养缺陷型菌株合成吡哆醇
Synthesis of Pyridoxine by a Pyridoxal Auxotroph of Escherichia coli.
作者信息
Dempsey W B
机构信息
Department of Biochemistry, College of Medicine, University of Florida, Gainesville, Florida.
出版信息
J Bacteriol. 1966 Aug;92(2):333-7. doi: 10.1128/jb.92.2.333-337.1966.
Abstract
Dempsey, Walter B. (University of Florida, Gainesville). Synthesis of pyridoxine by a pyridoxal auxotroph of Escherichia coli. J. Bacteriol. 92:333-337. 1966.-A pyridoxal auxotroph of Escherichia coli B produced pyridoxol and pyridoxol 5'-phosphate during starvation for pyridoxal. The identification of these compounds was made both by bioassay and by ion-exchange chromatography. Pyridoxol 5'-phosphate oxidase activity was absent in extracts of the auxotroph. The rate of synthesis of total pyridoxine by a pyridoxal-starved culture of this auxotroph was 6.0 x 10(-6) moles per mg per hr. Cellular content of pyridoxine was constant at 4.0 x 10(-10) moles/mg.
摘要
邓普西，沃尔特·B.（佛罗里达大学，盖恩斯维尔）。大肠杆菌的一种吡哆醛营养缺陷型合成吡哆醇。《细菌学杂志》92:333 - 337。1966年。——大肠杆菌B的一种吡哆醛营养缺陷型在缺乏吡哆醛的饥饿状态下产生吡哆醇和5'-磷酸吡哆醇。这些化合物通过生物测定和离子交换色谱法进行鉴定。该营养缺陷型的提取物中不存在5'-磷酸吡哆醇氧化酶活性。这种营养缺陷型在吡哆醛饥饿培养时，总吡哆醇的合成速率为每毫克每小时6.0×10⁻⁶摩尔。细胞内吡哆醇含量恒定在4.0×10⁻¹⁰摩尔/毫克。
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