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用于确定生物分子组装体中极性和质子性的自旋标记电子顺磁共振：跨膜分布

Spin-Label EPR for Determining Polarity and Proticity in Biomolecular Assemblies: Transmembrane Profiles.

作者信息

Marsh Derek

机构信息

Abteilung Spektroskopie, Max-Planck-Institut für biophysikalische Chemie, 37070 Göttingen, Germany.

出版信息

Appl Magn Reson. 2010 Jan;37(1-4):435-454. doi: 10.1007/s00723-009-0078-3. Epub 2009 Nov 17.

DOI:10.1007/s00723-009-0078-3
PMID:19960064
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2784069/
Abstract

Hyperfine couplings and g-values of nitroxyl spin labels are sensitive to polarity and hydrogen bonding in the environment probed. The dependences of these electronic paramagnetic resonance (EPR) properties on environmental dielectric permittivity and proticity are reviewed. Calibrations are given, in terms of the Block-Walker reaction field and local proton donor concentration, for the nitroxides that are commonly used in spin labeling of lipids and proteins. Applications to studies of the transverse polarity profiles in lipid bilayers, which constitute the permeability barrier of biological membranes, are reviewed. Emphasis is given to parallels with the permeation profiles of oxygen and nitric oxide that are determined from spin-label relaxation enhancements by using nonlinear continuous-wave EPR and saturation recovery EPR, and with permeation profiles of D(2)O that are determined by using (2)H electron spin echo envelope modulation spectroscopy.

摘要

硝酰自旋标记物的超精细耦合和g值对所探测环境中的极性和氢键敏感。本文综述了这些电子顺磁共振（EPR）性质对环境介电常数和质子性的依赖性。根据用于脂质和蛋白质自旋标记的常见氮氧化物的布洛赫-沃克反应场和局部质子供体浓度进行了校准。本文还综述了其在构成生物膜渗透屏障的脂质双层横向极性分布研究中的应用。重点介绍了与通过非线性连续波EPR和饱和恢复EPR由自旋标记弛豫增强确定的氧气和一氧化氮渗透分布以及通过2H电子自旋回波包络调制光谱确定的D(2)O渗透分布的相似之处。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/24903beff454/723_2009_78_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/5e76b0a09b32/723_2009_78_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/c9be45c4f07c/723_2009_78_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/af80cd8d79dc/723_2009_78_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/ad041d2c1fa1/723_2009_78_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/a0889d92ddc4/723_2009_78_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/24903beff454/723_2009_78_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/5e76b0a09b32/723_2009_78_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/5cb7ddd9368b/723_2009_78_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/4bac57c137fa/723_2009_78_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/c9be45c4f07c/723_2009_78_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/af80cd8d79dc/723_2009_78_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/ad041d2c1fa1/723_2009_78_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/a0889d92ddc4/723_2009_78_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23f5/2784069/24903beff454/723_2009_78_Fig8_HTML.jpg

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