Riccetto Cássio Luís Zanettini, Novaski Gustavo Lourenço, Palma Paulo César Rodrigues, Dambros Míriam, Thiel Marcelo, Vidal Benedicto de Campos
Division of Urology, State University of Campinas-UNICAMP, São Paulo, Brazil.
Urology. 2006 Apr;67(4):851-4. doi: 10.1016/j.urology.2005.10.043. Epub 2006 Mar 29.
To evaluate the effectiveness and safety of a new purified collagen gel with specific properties for renal artery embolization in an experimental model in dogs.
The new gel was prepared from type I bovine tendon collagen, highly purified to eliminate immunogenicity, and reconstituted through extensive dialysis in distilled water without the addition of glutaraldehyde. The study was conducted on 16 mongrel dogs that underwent random embolization of the renal artery. The dogs were divided into two groups: group 1 was killed after 2 days and group 2 after 21 days. The abdominal organs, lungs, and muscles of the posterior limbs were studied in detail and samples were taken for an anatomic-pathologic analysis.
After embolization, uniform parenchymatosus ischemia and loose perirenal adhesions without significant volumetric changes were observed in group 1. A microscopic study revealed acidophilus amorphous and birefringent emboli filling the lumen of the renal artery and extending into the arterioles. The glomeruli and tubules showed early signs of diffused necrosis. In group 2, microscopic examination revealed endothelial proliferation around the emboli that had closely adhered to the arterial wall, with signs of fibroblastic proliferation within the emboli. In both groups, the abdominal organs, lungs, and muscles of the posterior limbs did not show signs of collagen migration.
Purified collagen gel effectively embolized the renal artery. The absence of collagen migration to other organs and posterior limbs suggests that this material can be safely used for organ embolization.
在犬实验模型中评估一种具有特定特性的新型纯化胶原凝胶用于肾动脉栓塞的有效性和安全性。
新型凝胶由I型牛肌腱胶原制备而成，经过高度纯化以消除免疫原性，并在不添加戊二醛的情况下于蒸馏水中进行广泛透析后重构。对16只杂种犬进行肾动脉随机栓塞研究。将犬分为两组：第1组在2天后处死，第2组在21天后处死。对腹部器官、肺和后肢肌肉进行详细研究，并采集样本进行解剖病理学分析。
栓塞后，第1组观察到均匀的实质缺血和肾周疏松粘连，体积无明显变化。显微镜研究显示嗜酸性无定形和双折射栓子填充肾动脉腔并延伸至小动脉。肾小球和肾小管显示出早期弥漫性坏死迹象。在第2组中，显微镜检查显示栓子周围内皮细胞增生，栓子紧密粘附于动脉壁，栓子内有成纤维细胞增生迹象。两组中，腹部器官、肺和后肢肌肉均未显示胶原迁移迹象。
纯化胶原凝胶可有效栓塞肾动脉。胶原未迁移至其他器官和后肢表明该材料可安全用于器官栓塞。
