Elton D M, Bonass W A, Killington R A, Meredith D M, Halliburton I W
Department of Microbiology, University of Leeds, UK.
Am J Vet Res. 1991 Aug;52(8):1252-7.
The DNA fragments representing the entire short unique region and part of the repeat sequences of the equine herpesvirus type-1 genome were cloned into plasmid vectors. The approximate positions of the junctions between the short unique region and the inverted repeats were then located by restriction endonuclease mapping. Two open reading frames coding for potential glycoproteins have been identified within the short unique region, using DNA sequence analysis. The predicted amino acid sequences of these open reading frames had extensive homology to the herpes simplex virus glycoproteins gE and gI and the related glycoproteins of pseudorabies virus and varicella-zoster virus.
将代表马疱疹病毒1型基因组整个短独特区域和部分重复序列的DNA片段克隆到质粒载体中。然后通过限制性内切酶图谱定位短独特区域与反向重复序列之间连接的大致位置。利用DNA序列分析，在短独特区域内鉴定出两个编码潜在糖蛋白的开放阅读框。这些开放阅读框的预测氨基酸序列与单纯疱疹病毒糖蛋白gE和gI以及伪狂犬病病毒和水痘-带状疱疹病毒的相关糖蛋白具有广泛的同源性。
