Bonass W A, Hudson W A, Elton D M, Killington R A, Halliburton I W
Department of Microbiology, University of Leeds, U.K.
Arch Virol. 1994;134(1-2):169-78. doi: 10.1007/BF01379115.
Restriction enzyme digests of DNA from 22 unselected isolates of EHV-1 were analysed by hybridization with cloned DNA fragments covering the genome. In addition to a small amount of inter-strain variation, heterogeneity within strains was observed, caused by loss of specific restriction endonuclease sites in the DNA of a proportion of the virus particles of any one stock. Fifteen strains demonstrated the same intra-strain variation involving loss of the BamHI L-M site which was shown to lie within coding sequence for the large subunit of ribonucleotide reductase. This particular mutation may therefore be selected for by passage in RK13 cells.
用覆盖该基因组的克隆DNA片段进行杂交分析，对22株未经筛选的马疱疹病毒1型（EHV-1）分离株的DNA进行限制性酶切消化。除了少量的菌株间差异外，还观察到菌株内的异质性，这是由任一毒株的一部分病毒颗粒DNA中特定限制性内切酶位点的缺失引起的。15个菌株表现出相同的菌株内变异，涉及BamHI L-M位点的缺失，该位点位于核糖核苷酸还原酶大亚基的编码序列内。因此，这种特定的突变可能是在RK13细胞传代过程中被选择出来的。
