[高效液相色谱梯度洗脱法测定防风根中4种成分]
[Determination of four components in root of Saposhnikovia divaricata by HPLC gradient elution].
作者信息
Li Li, Liu Yuan-Yan, Geng Li-Dong, Xiao Yong-Qing
机构信息
Institute of Chinese Materia Media, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Feb;31(3):194-6.
PMID:16572995
Abstract
OBJECTIVE
To develop an HPLC method for determination of components in root of Saposhnikovia divaricata.
METHOD
The separation of four components was achieved on a reversed-phase Alltima C18 column with MeOH-H2O gradient elution at a flow rate of 1.0 mL x min(-1).
RESULT
Six samples were determined and there is no distinct difference between the contents determined by the new and old methods.
CONCLUSION
The new method is more simple and convenient than the old method.
摘要
目的
建立一种测定防风根中成分的高效液相色谱法。
方法
采用反相Alltima C18柱，以甲醇-水梯度洗脱，流速为1.0 mL·min⁻¹，实现了4种成分的分离。
结果
测定了6个样品，新方法与旧方法测定的含量之间无明显差异。
结论
新方法比旧方法更简便。
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