Wu Zhao-Hui, Luo Jia-Bo, Tan Xiao-Mei
Key laboratory of Research of Traditional Chinese Medicine and New Drug of Southern Medical University, Guangzhou 510515, China.
Zhongguo Zhong Yao Za Zhi. 2006 Feb;31(3):209-12.
To set up the HPLC fingerprint of Glycyrrhiza uralensis in decoctions prepared from various combinations of the recipe named Mahuang decoctions and study the influence of compatibility on fingerprint of G. wralensis in decoctions.
The RP-HPLC method was used, chromatography conditions were Zorbax SB-C18 column (4.6 mm x 250 mm, 5 microm), the acetonitrile-1.2% acetic acid (gradient elution) as mobile phase and detective wavelength at 254 nm.
The method is stable and reliable with a good reproducibility and provides a reference standard for the quality control of G. uralensis, it can be used to determine the fingerprint of G. aralensis in Mahuang decoctions. The result showed that 8 peaks were common in the fingerprint of G. uralensis in Mahauang decoctions. The area changes of 8 common peaks in decoctions prepared from various combinations of Mahuang decoctions were studied.
The fingerprint of G. uralensis in Mahuang decoctions is markedly influenced by herba ephedrae and ramulus cinnamomi and semen armeniacae amarum. The areas of 7 common peaks were reduced obviously, while the relative areas of 6 common peaks were increased remarkably.
建立麻黄汤类方不同配伍煎液中甘草的高效液相色谱指纹图谱，研究配伍对方中甘草指纹图谱的影响。
采用RP-HPLC法，色谱条件为Zorbax SB-C18柱（4.6 mm×250 mm，5μm），以乙腈-1.2%醋酸（梯度洗脱）为流动相，检测波长为254 nm。
该方法稳定可靠，重复性好，可为甘草质量控制提供参考标准，可用于测定麻黄汤类方中甘草的指纹图谱。结果显示，麻黄汤类方中甘草指纹图谱有8个共有峰。研究了麻黄汤类方不同配伍煎液中8个共有峰的面积变化。
麻黄、桂枝、苦杏仁对麻黄汤类方中甘草指纹图谱有显著影响。7个共有峰面积明显减小，6个共有峰相对面积显著增加。
