Kawahara Yukio, Nishikura Kazuko
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA.
FEBS Lett. 2006 Apr 17;580(9):2301-5. doi: 10.1016/j.febslet.2006.03.042. Epub 2006 Mar 24.
One type of RNA editing converts adenosine residues to inosine in double-stranded regions. Recent transcriptome analysis has revealed that numerous Alu repeats, present within introns and untranslated regions of human transcripts, are subject to this A-->I RNA editing. Furthermore, it revealed global transcription of antisense RNAs. Here, we demonstrate that antisense RNAs are also edited extensively but only in their Alu repeat sequences, and editing does not extend to the surrounding sequence. Our findings imply that sense and antisense RNAs form two separate intramolecular double-stranded RNAs consisting of inversely oriented Alu repeats, but rarely form intermolecular duplexes.
一种RNA编辑类型是在双链区域将腺苷残基转化为肌苷。最近的转录组分析表明，人类转录本的内含子和非翻译区域中存在的大量Alu重复序列会发生这种A→I RNA编辑。此外，它还揭示了反义RNA的整体转录情况。在这里，我们证明反义RNA也会被广泛编辑，但仅限于其Alu重复序列，且编辑不会延伸到周围序列。我们的研究结果表明，正义和反义RNA形成了由反向排列的Alu重复序列组成的两个独立的分子内双链RNA，但很少形成分子间双链体。
