Liu Yang, Yang Junshan, Cai Zongwei
Peking Union Medical College, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100094, China.
J Pharm Biomed Anal. 2006 Aug 28;41(5):1642-7. doi: 10.1016/j.jpba.2006.02.033. Epub 2006 Mar 29.
Sijunzi decoction consists of Panax ginseng, Poria cocos, Atractylodes macrocephala and Glycyrrhiza uralensis. High performance liquid chromatography coupled with tandem mass spectrometry (LC/MS(n)) was applied to identify and characterize three types of active components, ginsenoside (from P. ginseng), flavonoid and triterpenoid (from G. uralensis) in Sijunzi decoction. Spectra of MS and MS/MS from M+Na ions of ginsenosides were acquired and interpreted for their identification. Fragmentations with losing masses of 194 or 176Da were the characteristic ions of triterpenoids in the MS/MS analysis. A characteristic fragment ion of the aglycon moiety at m/z 257 from source collision-induced dissociation was observed for flavonoid. LC/MS was also applied for the comparison of relative peak area of major active components between Sijunzi decoction and the single herb extracts. The concentration ratios of major active components detected in the individual herbs of P. ginseng and G. uralensis were found different from those in Sijunzi decoction. The experimental data indicated that the decocting process could result in the difference in the amount of active components.
四君子汤由人参、茯苓、白术和甘草组成。采用高效液相色谱-串联质谱法（LC/MS(n)）对四君子汤中的三种活性成分进行鉴定和表征，即人参皂苷（来自人参）、黄酮类化合物和三萜类化合物（来自甘草）。获取并解释人参皂苷M+Na离子的质谱和二级质谱，以进行鉴定。在二级质谱分析中，失去194或176Da质量的碎片是三萜类化合物的特征离子。对于黄酮类化合物，观察到源碰撞诱导解离产生的m/z 257处苷元部分的特征碎片离子。LC/MS还用于比较四君子汤与单味药提取物中主要活性成分的相对峰面积。发现人参和甘草单味药中检测到的主要活性成分的浓度比与四君子汤中的不同。实验数据表明，煎煮过程可能导致活性成分含量的差异。
