• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

来自不同细菌的DNA中CC(A/T)GG序列（Dcm甲基化）中5-甲基胞嘧啶的存在情况。

Presence of 5-methylcytosine in CC(A/T)GG sequences (Dcm methylation) in DNAs from different bacteria.

作者信息

Gomez-Eichelmann M C, Levy-Mustri A, Ramirez-Santos J

机构信息

Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City.

出版信息

J Bacteriol. 1991 Dec;173(23):7692-4. doi: 10.1128/jb.173.23.7692-7694.1991.

DOI:10.1128/jb.173.23.7692-7694.1991
PMID:1657894
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC212538/
Abstract

The presence of CC(A/T)GG sequences with methylated internal cytosine (Dcm methylation) was determined in DNA from different genera of eubacteria. This methylation was studied by using restriction enzymes EcoRII and BstNI, which cleave unmethylated or methylated CC(A/T)GG sequences. Dcm methylation was only detected in genera of the family Enterobacteriaceae closely related to Escherichia: Shigella, Citrobacter, Salmonella, and Klebsiella.

摘要

在真细菌不同属的DNA中测定了带有甲基化内部胞嘧啶的CC(A/T)GG序列（Dcm甲基化）的存在情况。通过使用限制性内切酶EcoRII和BstNI来研究这种甲基化，这两种酶可切割未甲基化或甲基化的CC(A/T)GG序列。仅在与大肠杆菌密切相关的肠杆菌科各属中检测到Dcm甲基化：志贺氏菌属、柠檬酸杆菌属、沙门氏菌属和克雷伯氏菌属。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdb8/212538/8d2a9692f6e2/jbacter01041-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdb8/212538/8d2a9692f6e2/jbacter01041-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdb8/212538/8d2a9692f6e2/jbacter01041-0297-a.jpg

相似文献

1
Presence of 5-methylcytosine in CC(A/T)GG sequences (Dcm methylation) in DNAs from different bacteria.来自不同细菌的DNA中CC(A/T)GG序列（Dcm甲基化）中5-甲基胞嘧啶的存在情况。
J Bacteriol. 1991 Dec;173(23):7692-4. doi: 10.1128/jb.173.23.7692-7694.1991.
2
Determination of DNA sequences containing methylcytosine in Bacillus subtilis Marburg.枯草芽孢杆菌马伯格株中含甲基胞嘧啶的DNA序列的测定。
J Bacteriol. 1985 Aug;163(2):573-9. doi: 10.1128/jb.163.2.573-579.1985.
3
Detection of 5-methylcytosine in DNA sequences.DNA序列中5-甲基胞嘧啶的检测。
Nucleic Acids Res. 1978 May;5(5):1479-85. doi: 10.1093/nar/5.5.1479.
4
Restriction analysis and quantitative estimation of methylated bases of filamentous and unicellular cyanobacterial DNAs.丝状和单细胞蓝藻DNA的甲基化碱基的限制性分析和定量估计
J Bacteriol. 1988 Apr;170(4):1934-9. doi: 10.1128/jb.170.4.1934-1939.1988.
5
Bacterial DNA methylation and gene transfer efficiency.细菌DNA甲基化与基因转移效率。
Biochem Biophys Res Commun. 2000 Oct 5;276(3):1261-4. doi: 10.1006/bbrc.2000.3603.
6
Cleavage of methylated CCCGGG sequences containing either N4-methylcytosine or 5-methylcytosine with MspI, HpaII, SmaI, XmaI and Cfr9I restriction endonucleases.使用MspI、HpaII、SmaI、XmaI和Cfr9I限制性内切酶切割含有N4-甲基胞嘧啶或5-甲基胞嘧啶的甲基化CCCGGG序列。
Nucleic Acids Res. 1987 Sep 11;15(17):7091-102. doi: 10.1093/nar/15.17.7091.
7
DNA bending induced by DNA (cytosine-5) methyltransferases.DNA（胞嘧啶-5）甲基转移酶诱导的DNA弯曲
Nucleic Acids Res. 2000 Aug 15;28(16):3083-91. doi: 10.1093/nar/28.16.3083.
8
DNA methylation in the fungi.真菌中的DNA甲基化。
J Biol Chem. 1984 Jul 10;259(13):8033-6.
9
Mycoplasma restriction: identification of a new type of restriction specificity for DNA containing 5-methylcytosine.支原体限制作用：对含5-甲基胞嘧啶DNA的新型限制特异性的鉴定
J Bacteriol. 1986 Jan;165(1):219-25. doi: 10.1128/jb.165.1.219-225.1986.
10
DNA methylation in mycobacteria: absence of methylation at GATC (Dam) and CCA/TGG (Dcm) sequences.分枝杆菌中的DNA甲基化：GATC（Dam）和CCA/TGG（Dcm）序列处不存在甲基化。
FEMS Immunol Med Microbiol. 1995 Jul;11(4):291-6. doi: 10.1111/j.1574-695X.1995.tb00159.x.

引用本文的文献

1
Extension of bacterial rDNA sequencing for simultaneous methylation detection and its application in microflora analysis.细菌 rDNA 测序的扩展用于同时检测甲基化及其在微生物分析中的应用。
Sci Rep. 2023 Apr 7;13(1):5731. doi: 10.1038/s41598-023-28706-w.
2
Restriction Endonuclease-Based Modification-Dependent Enrichment (REMoDE) of DNA for Metagenomic Sequencing.基于限制内切酶修饰的宏基因组测序 DNA 富集（REMoDE）
Appl Environ Microbiol. 2023 Jan 31;89(1):e0167022. doi: 10.1128/aem.01670-22. Epub 2022 Dec 15.
3
DNA cytosine methylation at the lexA promoter of Escherichia coli is stationary phase specific.

本文引用的文献

1
THE ENZYMATIC METHYLATION OF RIBONUCLEIC ACID AND DEOXYRIBONUCLEIC ACID. V. PURIFICATION AND PROPERTIES OF THE DEOXYRIBONUCLEIC ACID-METHYLATING ACTIVITY OF ESCHERICHIA COLI.核糖核酸与脱氧核糖核酸的酶促甲基化作用。V. 大肠杆菌脱氧核糖核酸甲基化活性的纯化及性质
J Biol Chem. 1964 Nov;239:3858-65.
2
Sequence and substrate specificity of isolated DNA methylases from Escherichia coli C.来自大肠杆菌C的分离DNA甲基化酶的序列和底物特异性
J Bacteriol. 1983 Jan;153(1):274-80. doi: 10.1128/jb.153.1.274-280.1983.
3
Partially deficient methylation of cytosine in DNA at CCATGG sites stimulates genetic recombination of bacteriophage lambda.
大肠杆菌 lexA 启动子的 DNA 胞嘧啶甲基化是特定于停滞期的。
G3 (Bethesda). 2022 Feb 4;12(2). doi: 10.1093/g3journal/jkab409.
4
Differential gene expression profile of causing bacteremia in an immunocompromised individual.免疫功能低下个体中导致菌血症的差异基因表达谱。
Future Sci OA. 2020 Jan 29;6(4):FSO456. doi: 10.2144/fsoa-2019-0117.
5
Comparison of single-nucleotide variants identified by Illumina and Oxford Nanopore technologies in the context of a potential outbreak of Shiga toxin-producing Escherichia coli.在可能爆发产志贺毒素大肠杆菌的情况下，Illumina 和 Oxford Nanopore 技术鉴定的单核苷酸变异体的比较。
Gigascience. 2019 Aug 1;8(8). doi: 10.1093/gigascience/giz104.
6
Performance of neural network basecalling tools for Oxford Nanopore sequencing.基于神经网络的牛津纳米孔测序碱基调用工具的性能。
Genome Biol. 2019 Jun 24;20(1):129. doi: 10.1186/s13059-019-1727-y.
7
Methylation-Induced Hypermutation in Natural Populations of Bacteria.细菌自然种群中的甲基化诱导超突变。
J Bacteriol. 2018 Nov 26;200(24). doi: 10.1128/JB.00371-18. Print 2018 Dec 15.
8
Discovery of a bacterial 5-methylcytosine deaminase.一种细菌5-甲基胞嘧啶脱氨酶的发现。
Biochemistry. 2014 Dec 2;53(47):7426-35. doi: 10.1021/bi5012767. Epub 2014 Nov 19.
9
Genomic and proteomic characterization of the broad-host-range Salmonella phage PVP-SE1: creation of a new phage genus.广谱宿主范围沙门氏菌噬菌体 PVP-SE1 的基因组和蛋白质组特征：新噬菌体属的创建。
J Virol. 2011 Nov;85(21):11265-73. doi: 10.1128/JVI.01769-10. Epub 2011 Aug 24.
10
Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream production.质粒DNA疫苗载体设计：对效力、安全性及上游生产的影响
Biotechnol Adv. 2009 Jul-Aug;27(4):353-70. doi: 10.1016/j.biotechadv.2009.02.003. Epub 2009 Feb 20.
在CCATGG位点处，DNA中胞嘧啶的甲基化部分缺失会刺激噬菌体λ的基因重组。
Cell. 1982 Mar;28(3):531-41. doi: 10.1016/0092-8674(82)90208-2.
4
Absence in Bacillus subtilis and Staphylococcus aureus of the sequence-specific deoxyribonucleic acid methylation that is conferred in Escherichia coli K-12 by the dam and dcm enzymes.枯草芽孢杆菌和金黄色葡萄球菌中不存在由dam和dcm酶在大肠杆菌K-12中赋予的序列特异性脱氧核糖核酸甲基化。
J Bacteriol. 1981 Jul;147(1):259-61. doi: 10.1128/jb.147.1.259-261.1981.
5
DNA methylation and gene function.DNA甲基化与基因功能。
Science. 1980 Nov 7;210(4470):604-10. doi: 10.1126/science.6254144.
6
DNA adenine methylation of GATC sequences appeared recently in the Escherichia coli lineage.GATC序列的DNA腺嘌呤甲基化最近出现在大肠杆菌谱系中。
J Bacteriol. 1984 Nov;160(2):586-90. doi: 10.1128/jb.160.2.586-590.1984.
7
5-methylcytosine and 6-methylamino-purine in bacterial DNA.细菌DNA中的5-甲基胞嘧啶和6-甲基氨基嘌呤。
Nature. 1968 Jun 15;218(5146):1066-7. doi: 10.1038/2181066a0.
8
Genetic control of the secondary modification of deoxyribonucleic acid in Escherichia coli.大肠杆菌中脱氧核糖核酸二级修饰的遗传控制。
J Bacteriol. 1970 Oct;104(1):57-62. doi: 10.1128/jb.104.1.57-62.1970.
9
Isolation of deoxyribonucleic acid methylase mutants of Escherichia coli K-12.大肠杆菌K-12脱氧核糖核酸甲基化酶突变体的分离
J Bacteriol. 1973 Jun;114(3):1143-50. doi: 10.1128/jb.114.3.1143-1150.1973.
10
DNA methylation in Escherichia coli.大肠杆菌中的DNA甲基化
Annu Rev Genet. 1987;21:113-31. doi: 10.1146/annurev.ge.21.120187.000553.