Gomez-Eichelmann M C, Levy-Mustri A, Ramirez-Santos J
Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City.
J Bacteriol. 1991 Dec;173(23):7692-4. doi: 10.1128/jb.173.23.7692-7694.1991.
The presence of CC(A/T)GG sequences with methylated internal cytosine (Dcm methylation) was determined in DNA from different genera of eubacteria. This methylation was studied by using restriction enzymes EcoRII and BstNI, which cleave unmethylated or methylated CC(A/T)GG sequences. Dcm methylation was only detected in genera of the family Enterobacteriaceae closely related to Escherichia: Shigella, Citrobacter, Salmonella, and Klebsiella.
在真细菌不同属的DNA中测定了带有甲基化内部胞嘧啶的CC(A/T)GG序列(Dcm甲基化)的存在情况。通过使用限制性内切酶EcoRII和BstNI来研究这种甲基化,这两种酶可切割未甲基化或甲基化的CC(A/T)GG序列。仅在与大肠杆菌密切相关的肠杆菌科各属中检测到Dcm甲基化:志贺氏菌属、柠檬酸杆菌属、沙门氏菌属和克雷伯氏菌属。