评估一种商用夹心酶联免疫吸附测定法在诊断猎鹰曲霉病中的应用。

Assessment of a commercial sandwich ELISA in the diagnosis of aspergillosis in falcons.

作者信息

Arca-Ruibal B, Wernery U, Zachariah R, Bailey T A, Di Somma A, Silvanose C, McKinney P

机构信息

Dubai Falcon Hospital, PO Box 23919, Dubai, United Arab Emirates.

出版信息

Vet Rec. 2006 Apr 1;158(13):442-4. doi: 10.1136/vr.158.13.442.

DOI:10.1136/vr.158.13.442

PMID:16581995

Abstract

A commercial sandwich elisa (Platelia Aspergillus EIA; Bio-Rad) developed for the detection of galactomannan, a major cell wall constituent of Aspergillus species, was tested for its efficacy in the diagnosis of aspergillosis in falcons. Ninety serum samples from 50 aspergillosis-positive falcons and 182 samples from 142 aspergillosis-negative falcons were tested. The sensitivity of the test was only 12 per cent and its specificity was 95 percent. The test was therefore unsatisfactory for detecting galactomannan in the serum samples and cannot be used as a screening test for aspergillosis in falcons.

摘要

一种用于检测半乳甘露聚糖（曲霉属主要细胞壁成分）的商用夹心酶联免疫吸附测定法（Platelia Aspergillus EIA；伯乐公司），针对其在诊断猎鹰曲霉病中的功效进行了测试。对来自50只曲霉病阳性猎鹰的90份血清样本以及来自142只曲霉病阴性猎鹰的182份样本进行了检测。该检测的灵敏度仅为12%，特异性为95%。因此，该检测在血清样本中检测半乳甘露聚糖方面并不理想，不能用作猎鹰曲霉病的筛查检测。